Role of LGI1 in Autosomal Dominant Lateral Temporal Lobe Epilepsy

LGI1 在常染色体显性遗传性外侧颞叶癫痫中的作用

• 批准号: 7525735
• 负责人: MATTHEW P ANDERSON
• 金额: $ 33.47万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-18 至 2012-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7525735
• 关键词: 2-tyrosine; 3-Dimensional; ADAM Family Protein; Acute; Address; Afferent Neurons; Animals; Antibodies; Auditory; Axon; Bacterial Artificial Chromosomes; Bacterial Genes; Binding; Brain; Brain Diseases; Cells; Chromosome Pairing; Complex; Computer software; Count; Cytoplasmic Granules; DLG4 gene; DNA; Data; Dendrites; Dependence; Development; Dimerization; Disease; Dominant-Negative Mutation; Down-Regulation; Engineering; Epilepsy; Figs - dietary; Frequencies; Gene Expression; Genes; Genomics; Genotype; Glioma; Glutamate Receptor; Glutamates; Goals; Haploidy; Hippocampus (Brain); Human; Immunoprecipitation; In Vitro; Inferior Colliculus; Inherited; Integrins; Ion Channel; LGI1 gene; Lateral; Learning; Length; Leucine; Life; Link; Localized; Long-Term Potentiation; Measures; Medial; Mediating; Membrane; Methods; Modeling; Molecular; Mus; Mutate; Mutation; N-Methyl-D-Aspartate Receptors; N-Methylaspartate; NR2B NMDA receptor; Neurons; Numbers; PTK2 gene; Pathway interactions; Pattern; Perforant Pathway; Physiologic pulse; Potassium Channel; Probability; Process; Property; Protein Binding; Protein Kinase; Protein Tyrosine Kinase; Protein-Serine-Threonine Kinases; Proteins; Public Health; Pulse taking; RNA Splicing; Receptor Down-Regulation; Research; Role; Sensory; Series; Signal Pathway; Signal Transduction; Slice; Staining method; Stains; Structure of molecular layer of cerebellar cortex; Synapses; Synaptic Transmission; Synaptic Vesicles; Synaptic plasticity; Temporal Lobe Epilepsy; Terminator Codon; Testing; Thalamic structure; Time; Transcript; Transgenic Mice; Transgenic Organisms; Vesicle; Western Blotting; base; biocytin; dendrotoxin; density; dentate gyrus; dimer; early childhood; entorhinal cortex; gamma-Aminobutyric Acid; human LGI1 protein; in vivo; inhibitor/antagonist; insight; kinase inhibitor; mutant; nerve supply; neural circuit; novel; patch clamp; postnatal; postsynaptic; presynaptic; prevent; protein expression; receptor; receptor function; reconstruction; response; src-Family Kinases; therapeutic target; transmission process

DESCRIPTION (provided by applicant): A recently discovered human epilepsy gene, LGI1 (leucine-rich glioma-inactivated; mutated to cause human autosomal dominant lateral temporal lobe epilepsy or ADLTE) encodes a protein secreted at glutamate synapses during postnatal glutamate synapse development. Consequently, we hypothesize that LGI1 might promote epilepsy through a novel mechanism, by regulating postnatal glutamate synapse maturation. We propose ADLTE mutant LGI1 acts as a dominant negative to inhibit native LGI1 function and arrest maturation. To directly address our hypothesis and contrast the functional effects of epilepsy-associated mutant LGI1 with those of excess wild-type LGI1 on native neural circuits, we created transgenic mice using bacterial artificial chromosomes (BAC) carrying a large 226 kb fragment of mouse genomic DNA encoding the full-length LGI1 gene. The ADLTE 835delC mutation introduced a premature translational stop codon to generate a truncated LGI1 protein. The full-length gene BAC transgenic approach is important to maintain native patterns of gene expression and transcript splicing in order to assess the genes effects on the glutamate synapse development process in vivo. LGI1 is heavily expressed presynaptically at medial entorhinal cortex perforant pathway glutamate synapses innervating dentate granule neurons (MPP- dentate) and also separately in a synapse targeting thalamus. Our overall goal is to define the cellular basis for human ADLT epilepsy. We specifically test whether excess LGI1 magnifies and mutant LGI1 blocks maturation of the following glutamate synapse properties during the postnatal periods when it becomes expressed and throughout adulthood in dentate gyrus and thalamus. We examine: 1) presynaptic glutamate release down-regulation and role of Kv1.1 K+ channel activity; 2) postsynaptic NR2B NMDA receptor current and synaptic plasticity down-regulation, PSD95-Src complex formation, and the role of Src kinase activity; and 3) dendrite branch (e.g., dentate) and axon input (e.g., thalamus) pruning. This murine model of human ADLT epilepsy could link a novel human epilepsy gene to the arrest of glutamate synapse maturation and potentially defining a cellular basis for human seizure disorders beyond ADLTE. PUBLIC HEALTH RELEVANCE: This project seeks to identify the cellular basis of the inherited human epilepsy disorder caused by mutations in LGI1, a secreted synaptic protein. The results should provide new insights into the cellular and molecular basis of human epilepsy, and help identify new potential therapeutic targets to treat this common brain disorder.

描述（由申请人提供）：最近发现的人类癫痫基因LGI 1（富含亮氨酸的神经胶质瘤失活;突变导致人类常染色体显性外侧颞叶癫痫或ADLTE）编码一种在出生后谷氨酸突触发育期间在谷氨酸突触分泌的蛋白质。因此，我们假设LGI 1可能通过调节出生后谷氨酸突触成熟的新机制促进癫痫。我们建议ADLTE突变体LGI 1作为显性负抑制天然LGI 1功能和逮捕成熟。为了直接解决我们的假设和对比癫痫相关突变体LGI 1与过量野生型LGI 1对天然神经回路的功能影响，我们使用携带编码全长LGI 1基因的小鼠基因组DNA的226 kb大片段的细菌人工染色体（BAC）创建了转基因小鼠。ADLTE 835 delC突变引入了提前翻译终止密码子以产生截短的LGI 1蛋白。全长基因BAC转基因方法对于维持基因表达和转录本剪接的天然模式以评估基因对体内谷氨酸突触发育过程的影响是重要的。LGI 1在突触前大量表达于内侧内嗅皮层穿通通路支配齿状颗粒神经元（MPP-齿状）的谷氨酸突触处，并且还单独地在靶向丘脑的突触中表达。我们的总体目标是确定人类ADLT癫痫的细胞基础。我们专门测试是否过量LGI 1放大和突变LGI 1块成熟的谷氨酸突触特性在出生后的时期，当它成为表达和整个成年期在齿状回和丘脑。我们检查：1）突触前谷氨酸释放下调和Kv1.1 K+通道活性的作用; 2）突触后NR 2B NMDA受体电流和突触可塑性下调、PSD 95-Src复合物形成和Src激酶活性的作用; 3）树突分支（例如，齿状）和轴突输入（例如，丘脑）修剪。这种人类ADLT癫痫的小鼠模型可以将一种新的人类癫痫基因与谷氨酸突触成熟的阻滞联系起来，并可能定义ADLTE以外的人类癫痫发作疾病的细胞基础。 公共卫生相关性：该项目旨在确定由分泌性突触蛋白LGI 1突变引起的遗传性人类癫痫疾病的细胞基础。这些结果将为人类癫痫的细胞和分子基础提供新的见解，并有助于确定治疗这种常见脑部疾病的新的潜在治疗靶点。