权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

EWOD-MALDI-MS for High-Throughput Proteomics

用于高通量蛋白质组学的 EWOD-MALDI-MS

基本信息

批准号：
7489875
负责人：
Robin L. Garrell
金额：
$ 31.93万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-09-06 至 2010-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7489875
关键词：
Address Adsorption Area Biology Cataloging Catalogs Charge Chemicals Chemistry Complex Detection Development Device Designs Devices Disease Disease Marker Electrodes Fingerprint Generations Liquid substance Location Mass Spectrum Analysis Methods Microfluidic Microchips Microfluidics Numbers Organic solvent product Particulate Peptides Phase Preparation Procedures Process Protein Array Proteins Proteomics Protocols documentation Range Reagent Research Research Personnel Rest Robin bird Saliva Sampling Solid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Standards of Weights and Measures Surface Surface Tension System Techniques Technology Testing Two-Dimensional Gel Electrophoresis Variant Water Work base design drug discovery electric field improved insight instrument ionization technique method development programs protein structure research study saliva analysis salivary diagnostics structural biology tool voltage

项目摘要

Proteomics research requires methods and instruments that can collect, store, catalogue and analyze vast amounts of information. To accelerate progress in proteomics research, we propose to develop electrowetting-on-dielectric (EWOD)-based microfluidics that will enable sample processing steps to be integrated with creation of high-throughput arrays for matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). In EWOD, electric fields are used to manipulate liquid surface tension. By applying a sequence of potentials across electrodes fabricated under a dielectric layer, droplets can be created from a reservoir, moved to specific locations, joined, mixed and divided. EWOD-based microfluidic devices are reconfigurable, and can handle neutral and charged analytes and particulates, in water or organic solvents. The research plan addresses five objectives: 1) EWOD device design and fabrication; 2) chracterization of EWOD as a sample preparation tool for MALDI-MS; 3) EWOD-based sample purification and processing; 4) integration of EWOD-MALDI-MS with micro-HPLC and CD separations, using a new method, "valet parking MALDI," and 5) two applications of EWOD-MALDI-MS: (a) detection of disease markers in saliva, and (b) generation of protein arrays for functional proteomics. Because it has the protential to integrate many sample processing steps with creation of high-throughput arrays for MALDI-MS, the proposed method will represent a major advance over existing technologies for proteomics. More broadly, the EWOD-based devices and sample processing protocols we develop will be applicable in a wide range of array-based methods in bioanalytical chemistry anad chemical biology.

蛋白质组学研究需要能够收集、存储、编目和分析大量蛋白质的方法和仪器大量的信息。为了加快蛋白质组学研究的进展，我们建议开发基于电介质上电润湿（EWOD）的微流体，这将使样品处理步骤能够与用于基质辅助激光解吸/电离质谱的高通量阵列的创建相结合质谱法（MALDI-MS）。在EWOD中，电场用于操纵液体表面张力。通过通过在电介质层下制造的电极上施加一系列电势，从水库中产生，移动到特定的位置，加入，混合和分裂。基于EWOD的微流控装置是可重新配置的，并且可以处理水中或水中的中性和带电分析物和颗粒，有机溶剂本研究计画包含五个目标：1）EWOD装置的设计与制作; 2） EWOD作为MALDI-MS样品制备工具的表征; 3）基于EWOD的样品纯化 4）EWOD-MALDI-MS与微型HPLC和CD分离的集成，使用新的方法，“代客泊车MALDI”，和5）EWOD-MALDI-MS的两个应用：（a）疾病检测唾液中的标志物，和（B）产生用于功能蛋白质组学的蛋白质阵列。因为它有潜力为了将许多样品处理步骤与MALDI-MS的高通量阵列的创建相结合，所提出的方法将代表现有蛋白质组学技术的重大进步。更广泛地说，我们开发的基于EWOD的设备和样品处理协议将适用于广泛的生物分析化学和化学生物学中的基于阵列的方法。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Incubated protein reduction and digestion on an electrowetting-on-dielectric digital microfluidic chip for MALDI-MS.

DOI：
10.1021/ac101833b
发表时间：
2010-12-01
期刊：
ANALYTICAL CHEMISTRY
影响因子：
7.4
作者：
Nelson, Wyatt C.;Peng, Ivory;Lee, Geun-An;Loo, Joseph A.;Garrell, Robin L.;Kim, Chang-Jin CJ
通讯作者：
Kim, Chang-Jin CJ

Dynamic contact angles and hysteresis under electrowetting-on-dielectric.