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CHARACTERIZATION OF PEPTIDE-SURFACE INTERACTIONS

CHARACTERIZATION OF PEPTIDE-SURFACE INTERACTIONS
肽-表面相互作用的表征
批准号:
3467858
负责人:
Robin L. Garrell
金额:
$8.6万
依托单位国家:
美国
项目类别:
财政年份:
1990
资助国家:
美国
项目状态:
已结题
起止时间:
1990-07-01 至 1995-06-30

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项目成果

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中文摘要
翻译
生物分子与表面的相互作用对药物的疗效至关重要 生物分析分离、生物传感器和假体设备。尽管 它们的重要性,人们对基础化学和 生物分子与表面之间的物理相互作用。的目标是 建议的研究是描述非共价相互作用的特征 金属表面的多肽。这项建议描述了系统的研究 旨在确定特定官能团和构象如何 氨基酸、多肽和小多肽的限制控制着 这些生物分子的吸附、取向和表面相互作用。 这些结果将极大地扩展我们对金属多肽的理解。 相互作用,以及我们对吸附行为的理解 溶液-固体界面上的多官能团分子。 多肽是蛋白质的极好的化学和光谱模型。我们 因此将使用多肽来确定 确定特定官能团和构象限制条件 多肽和蛋白质与蛋白质的吸光度和表面相互作用 非反应性表面。用于非共价吸附表面的研究 所有衬底共有的相互作用,贵金属几乎 理想的模型曲面。它们的表面化学相对简单 与石英、二氧化硅和聚合物相比。表面净电荷 并且疏水性可以简单地通过改变施加的电势来改变, 支持电解液和金属本身。 在这些研究中将使用的主要工具是拉曼和红外 光谱学,特别强调表面增强拉曼 光谱学(SERS)因其独特的灵敏度和选择性 吸附在贵金属表面。我们的SERS初步结果 论证了这种方法的可行性,并揭示了多肽 吸收受特定官能团-表面相互作用的支配 规定了在溶液中的吸附取向和构象- 金属接口。特别是,结果表明,多肽之间存在相互作用。 通过端胺基团和芳香基团与银强烈结合 侧链,特别是酪氨酸,在拟议的工作中,我们将 表征这些和其他多肽官能团-表面相互作用 并评价了它们对多肽的相对贡献 吸收率。次要目标是确定表面如何 电荷、疏水性和粗糙度影响多肽和多肽 吸附。
英文摘要
Biomolecule-surface interactions are essential to the efficacy of bioanalytical separations, biosensors, and prosthetic devices. Despite their importance, very little is known about fundamental chemical and physical interactions between biomolecules and surfaces. The goal of the proposed research is to characterize the non-covalent interactions of peptides with metal surfaces. This proposal describes systematic studies aimed at determining how specific functional groups and conformational constraints in amino acids, peptides, and small polypeptides control the adsorption, orientation, and surface interactions of these biomolecules. The results will significantly extend our understanding of peptide-metal interactions, and our understanding of the adsorption behavior of polyfunctional molecules at solution-solid interfaces. Peptides are excellent chemical and spectroscopic models for proteins. We will therefore use peptides to determine the relative importance of specific functional groups and conformational constraints in determining the absorptivity and surface interactions of peptides and proteins with non-reactive surfaces. For studies of the non-covalent adsorbate-surface interactions that are common to all substrates, noble metals are nearly ideal model surfaces. Their surface chemistry is relatively simple compared with that of quartz, silica, and polymers. The net surface charge and hydrophobicity can be varied simply by changing the applied potential, the supporting electrolyte, and the metal itself. The primary tools that will be used in these studies are Raman and infrared spectroscopy, with particular emphasis on surface-enhanced Raman spectroscopy (SERS) because of its unique sensitivity and selectivity for adsorbates on noble metal surfaces. Our preliminary SERS results demonstrate the feasibility of this approach, and reveal that peptide absorption is governed by specific functional group-surface interactions that prescribe the adsorbate orientation and conformation at the solution- metal interface. In particular, the results suggest that peptides interact strongly with silver through the terminal amine group and through aromatic side chains, particularly tyrosine, In the proposed work we will characterize these and other peptide functional group-surface interactions in detail, and evaluate their relative contributions to peptide absorptivity. A secondary objective is to determine how the surface charge, hydrophobicity, and roughness affect peptide and polypeptide adsorption.
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CHARACTERIZATION OF PEPTIDE-SURFACE INTERACTIONS
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