OPTIMIZATION OF METHODS FOR PRODUCTION OF BOTH ICSI- AND SCNT DERIVED BABOON

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。对于该方案，将用药物人工刺激狒狒产生卵子，这些卵子将通过微创腹部手术（腹腔镜检查）或超声引导抽吸收集。这些卵子将在实验室中使用新鲜或冷冻的精子进行受精。一旦受精，胚胎将被培养到早期细胞分裂阶段，以获得狒狒干细胞。一些卵子可能会被转移以产生克隆胚胎。这些胚胎也将被培养到早期细胞分裂阶段以获得干细胞。我们还将在激素刺激和时间交配后收集雌性的胚胎。这些胚胎通过用培养基冲洗子宫经宫颈收集。这些胚胎也将被培养，然后用于干细胞衍生。 我们预计，这项研究将使我们能够优化狒狒胚胎干细胞的衍生方法。任何已建立的品系都将接受严格的测试，以确定其效力和正常性。虽然人类胚胎干细胞可能具有令人难以置信的治疗价值，用于治疗广泛的疾病，如帕金森氏症、糖尿病、自闭症、心脏病、阿尔茨海默氏症等，在任何这样的试验开始之前，必须在动物模型中进行严格的测试。狒狒提供了一种有价值的非人灵长类动物模型，用于确定免疫匹配干细胞的安全性和有效性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For this protocol, baboons will be artificially stimulated with drugs to produce eggs that will be collected via minimally invasive abdominal surgery (laparoscopy) or ultrasound-guided aspiration. These eggs will be fertilized in the laboratory using fresh or frozen sperm. Once fertilized, the embryos will be cultured to the early cell division stage in order to derive baboon stem cells. Some eggs may undergo transfer to create cloned embryos. These embryos will also be cultured to the early cell division stage to derive stem cells. We will also collect embryos from females after hormonal stimulation and time-mating. These embryos are collected transcervically by flushing the uterus with medium. These embryos will also be cultured and then use for stem cell derivations. We anticipate that this research will enable us to optimize methods for the derivation of baboon embryonic stem cells. Any established lines will undergo rigorous testing to determine their potency and normalcy. While human embryonic stem cells may have incredible therapeutic value for treating a wide range of disorders such as Parkinson's diabetes, autism, heart disease, Alzheimer's etc., rigorous testing in animal models is necessary before any such trials can begin. Baboons provide a valuable non-human primate model in which to determine the safety and efficacy of immune-matched stem cells.