OPTIMIZATION OF METHODS FOR PRODUCTION OF BOTH ICSI- AND SCNT DERIVED BABOON

ICSI 和 SCNT 衍生狒狒生产方法的优化

• 批准号: 8357649
• 负责人: GERALD SCHATTEN
• 金额: $ 76.04万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357649
• 关键词: Abdomen; Alzheimer' s Disease; Animal Model; Autistic Disorder; Cell division; Derivation procedure; Diabetes Mellitus; Disease; Embryo; Embryo Cloning; Female; Flushing; Freezing; Funding; Grant; Heart Diseases; Hormonal; Immune; Intracytoplasmic Sperm Injections; Laboratories; Laparoscopy; Methods; Modeling; National Center for Research Resources; Normalcy; Operative Surgical Procedures; Papio; Parkinson Disease; Partner in relationship; Pharmaceutical Preparations; Primates; Principal Investigator; Production; Protocols documentation; Research; Research Infrastructure; Resources; Safety; Source; Staging; Stem cells; Testing; Therapeutic; Time; Ultrasonography; United States National Institutes of Health; Uterus; cost; egg; embryonic stem cell; human embryonic stem cell; minimally invasive; nonhuman primate; sperm cell

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. For this protocol, baboons will be artificially stimulated with drugs to produce eggs that will be collected via minimally invasive abdominal surgery (laparoscopy) or ultrasound-guided aspiration. These eggs will be fertilized in the laboratory using fresh or frozen sperm. Once fertilized, the embryos will be cultured to the early cell division stage in order to derive baboon stem cells. Some eggs may undergo transfer to create cloned embryos. These embryos will also be cultured to the early cell division stage to derive stem cells. We will also collect embryos from females after hormonal stimulation and time-mating. These embryos are collected transcervically by flushing the uterus with medium. These embryos will also be cultured and then use for stem cell derivations. We anticipate that this research will enable us to optimize methods for the derivation of baboon embryonic stem cells. Any established lines will undergo rigorous testing to determine their potency and normalcy. While human embryonic stem cells may have incredible therapeutic value for treating a wide range of disorders such as Parkinson's diabetes, autism, heart disease, Alzheimer's etc., rigorous testing in animal models is necessary before any such trials can begin. Baboons provide a valuable non-human primate model in which to determine the safety and efficacy of immune-matched stem cells. SNPRC is supplying baboons for this project.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 根据这一方案，将用药物人工刺激狒狒产生卵子，这些卵子将通过微创腹部手术(腹腔镜术)或超声波引导的吸入术收集。这些卵子将在实验室使用新鲜或冷冻的精子受精。一旦受精，胚胎将被培养到细胞分裂的早期阶段，以获得狒狒干细胞。一些卵子可能会被移植来创造克隆胚胎。这些胚胎也将被培养到细胞分裂的早期阶段，以获得干细胞。我们还将收集荷尔蒙刺激和时间交配后雌性的胚胎。这些胚胎是通过用培养液冲洗子宫来经宫颈收集的。这些胚胎也将被培养，然后用于干细胞的衍生。 我们预计，这项研究将使我们能够优化狒狒胚胎干细胞的提取方法。任何已建立的品系都将经过严格的测试，以确定它们的效力和正常性。虽然人类胚胎干细胞在治疗帕金森氏症、自闭症、心脏病、阿尔茨海默氏症等多种疾病方面可能具有令人难以置信的治疗价值，但在开始任何此类试验之前，有必要在动物模型中进行严格的测试。狒狒提供了一个有价值的非人类灵长类动物模型，在这个模型中可以确定免疫匹配干细胞的安全性和有效性。SNPRC正在为这个项目提供狒狒。