TOWARDS A SINGLE INJECTION METHOD OF DETERMINING IN VIVO PH BY MRI

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。以非侵入性方式测定体内pH值的能力将代表着肿瘤学的重大进步。磁共振造影剂现在被广泛使用，但目前临床使用的造影剂对生物信息不敏感，如离子浓度、酶活性或特定结合部位的存在。尽管能够报告PH值的试剂已经知道很多年了，但没有人超越了几个小动物的成像研究。在设计一个用核磁共振测量活体pH值的系统时，面临的最重要的问题是必须知道造影剂的浓度才能生成pH图。我们将在这个项目中开发一个简单、方便的解决方案来解决这个问题。本研究的目标是在这个系统中增加一个浓度标记成分，以提供一种单一的可注射试剂溶液，该溶液将在单一成像研究中产生组织细胞外pH的图像地图。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The capability to determine in vivo pH in a non-invasive manner would represent a major advance in oncology. MRI contrast media are now widely used but the agents currently in clinical use are insensitive to biological information such as changes in ion concentrations, enzyme activity or the presence of specific binding sites. Despite the fact that agents capable of reporting pH have been known for a number of years, none have gone beyond a few small animal imaging studies. The most significant problem faced when designing a system to measure pH in vivo by MRI is that the concentration of the contrast agent must be known in order to generate a pH map. We will develop a simple, convenient solution to this problem in this project. The goal of the present research is to add a concentration marker component to this system to provide a single injectable agent solution that will yield an image map of extracellular pH of tissue in a single imaging study.