Structural and functional analysis of a novel proline-rich dimerisation/oligomerisation domain
新型富含脯氨酸的二聚/寡聚结构域的结构和功能分析
基本信息
- 批准号:BB/D005094/1
- 负责人:
- 金额:$ 31.99万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Research Grant
- 财政年份:2006
- 资助国家:英国
- 起止时间:2006 至 无数据
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Proteins are long chains of units called amino acids and are essential components of all living things. Genes are segments of DNA that encode information on how to make the proteins that are required by the cell. This information is decoded by a set of proteins known as the transcription complex and the first step in the decoding process is known as transcription. To ensure that the information encoded by a gene is only transcribed in the correct circumstances there are a group of proteins called transcription factors that regulate the transcription process. Transcription factors are made up of a large and variable number of functional modules that allow them to interact with each other, with the transcription complex and with DNA. One group of modules that occur frequently both in transcription factors and also in a variety of other proteins is proline-rich. These modules are characterised by the presence of a large number of proline residues. Proline residues have a unique structure that cannot form many of the associations that occur between the other amino acids. One consequence of the unusual structure of proline is that proteins that contain a high density of proline residues are folded into their three dimensional shape (structure) in a way that is only poorly understood. The reason for having so many proline-rich modules in transcription factors and the structure of these modules are only poorly understood. The Proline-Rich Homeodomain (PRH) protein is a transcription factor that contains a high density of proline residues clustered in one region of the protein. PRH is an important protein in determining how cells divide and what sort of cells they will become in the future. The proline-rich region of PRH is important for regulating the transcription of genes, and for regulating cell division. Our present results show that the proline-rich region of PRH can bind to DNA and also to itself either to form small complexes called dimers that contain 2 PRH proteins or large complexes called oligomers that contain many PRH proteins. We want to understand how the proline-rich sequence in PRH influences the dimerisation and oligomerisation of the entire PRH protein. We also want to understand whether the ability of the PRH to bind to itself and to DNA using the proline-rich region is important for regulating transcription. Since other transcription factors that function in a similar manner to PRH also have similar proline-rich sequences, the information that we learn about PRH is very likely to be applicable to many other proteins.
蛋白质是被称为氨基酸的长链单位,是所有生物的基本组成部分。基因是DNA的片段,它编码如何制造细胞所需的蛋白质的信息。这些信息由一组被称为转录复合体的蛋白质解码,解码过程的第一步被称为转录。为了确保基因编码的信息只在正确的情况下转录,有一组被称为转录因子的蛋白质调节转录过程。转录因子由大量可变的功能模块组成,这些功能模块使它们能够相互作用,与转录复合体和DNA相互作用。在转录因子和各种其他蛋白质中经常出现的一组模块是富含脯氨酸的。这些模块的特点是存在大量的脯氨酸残基。脯氨酸残基具有独特的结构,不能形成其他氨基酸之间发生的许多结合。脯氨酸不寻常结构的一个后果是,含有高密度脯氨酸残基的蛋白质以一种人们知之甚少的方式折叠成三维形状(结构)。转录因子中有这么多富含脯氨酸的模块的原因以及这些模块的结构目前还不太清楚。脯氨酸富同源结构域(PRH)蛋白是一种转录因子,包含高密度的脯氨酸残基聚集在蛋白质的一个区域。PRH是一种重要的蛋白质,它决定细胞如何分裂,以及它们将来会变成什么样的细胞。PRH富含脯氨酸的区域对调控基因转录和细胞分裂具有重要作用。我们目前的研究结果表明,PRH富含脯氨酸的区域既可以与DNA结合,也可以与自身结合,形成含有2个PRH蛋白的小复合体,称为二聚体,也可以形成含有许多PRH蛋白的大复合体,称为低聚体。我们想了解PRH中富含脯氨酸的序列如何影响整个PRH蛋白的二聚化和寡聚化。我们还想了解PRH利用富含脯氨酸的区域与自身和DNA结合的能力对调节转录是否重要。由于与PRH功能相似的其他转录因子也有类似的富含脯氨酸的序列,我们了解到的关于PRH的信息很可能适用于许多其他蛋白质。
项目成果
期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
DNA compaction by the higher-order assembly of PRH/Hex homeodomain protein oligomers.
- DOI:10.1093/nar/gkq659
- 发表时间:2010-11
- 期刊:
- 影响因子:14.9
- 作者:Soufi A;Sawasdichai A;Shukla A;Noy P;Dafforn T;Smith C;Jayaraman PS;Gaston K
- 通讯作者:Gaston K
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Kevin Gaston其他文献
Protein kinase CK2 inhibition suppresses neointima formation via a proline-rich homeodomain-dependent mechanism
蛋白激酶 CK2 抑制通过富含脯氨酸的同源结构域依赖性机制抑制新内膜形成
- DOI:
- 发表时间:
2017 - 期刊:
- 影响因子:4
- 作者:
Kerry S. Wadey;B. A. Brown;G. Sala;Padma;Kevin Gaston;Sarah J. George - 通讯作者:
Sarah J. George
Interconversion of the DNA‐binding specificities of two related transcription regulators, CRP and FNR
两个相关转录调节因子 CRP 和 FNR 的 DNA 结合特异性的相互转换
- DOI:
- 发表时间:
1990 - 期刊:
- 影响因子:3.6
- 作者:
Stephen Spiro;Kevin Gaston;A. Bell;R. E. Roberts;S. Busby;J. R. Guest - 通讯作者:
J. R. Guest
DNA Binding and Bending by the Human Papillomavirus Type 16 E2 Protein: RECOGNITION OF AN EXTENDED BINDING SITE
- DOI:
10.1074/jbc.272.13.8236 - 发表时间:
1997-03-28 - 期刊:
- 影响因子:
- 作者:
Alison Thain;Kenneth Webster;Dave Emery;Anthony R. Clarke;Kevin Gaston - 通讯作者:
Kevin Gaston
Myc and YY1 mediate activation of the Surf-1 promoter in response to serum growth factors.
Myc 和 YY1 介导 Surf-1 启动子响应血清生长因子的激活。
- DOI:
10.1016/s0167-4781(00)00116-0 - 发表时间:
2000 - 期刊:
- 影响因子:0
- 作者:
Ellen G Vernon;Kevin Gaston - 通讯作者:
Kevin Gaston
Kevin Gaston的其他文献
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{{ truncateString('Kevin Gaston', 18)}}的其他基金
Nature and extent of ecological impacts of vehicle headlights
车辆前灯生态影响的性质和程度
- 批准号:
NE/Z000114/1 - 财政年份:2024
- 资助金额:
$ 31.99万 - 项目类别:
Research Grant
Artificial light as a driver of nighttime landscape ecology
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NE/V000497/1 - 财政年份:2021
- 资助金额:
$ 31.99万 - 项目类别:
Research Grant
Mapping ecological risks from the colour spectrum of artificial nighttime lighting using astronaut images of the earth
使用地球的宇航员图像从人造夜间照明的色谱中绘制生态风险图
- 批准号:
NE/P01156X/1 - 财政年份:2017
- 资助金额:
$ 31.99万 - 项目类别:
Research Grant
Effects of artificial light on multi-trophic population dynamics
人造光对多营养种群动态的影响
- 批准号:
NE/N001672/1 - 财政年份:2016
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$ 31.99万 - 项目类别:
Research Grant
Fragments, functions and flows - the scaling of biodiversity and ecosystem services in urban ecosystems
碎片、功能和流动——城市生态系统中生物多样性和生态系统服务的扩展
- 批准号:
NE/J015237/1 - 财政年份:2012
- 资助金额:
$ 31.99万 - 项目类别:
Research Grant
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