LOOKING FOR RNA CARGOS REGULATED BY FMRP

寻找受 FMRP 监管的 RNA 货物

• 批准号: 7358006
• 负责人: WILLIAM T GREENOUGH
• 金额: $ 1.26万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-29 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358006
• 关键词: RNA; RNA binding protein; amine oxidase (flavin); fragile X syndromes; genetically modified animals; human; intracellular transport; laboratory mouse; mental retardation; neurons; proteins

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. FMRP is the protein mutated from fragile X syndrome, which is a common mental retardation in human. FMRP is also a RNA binding protein and may regulate RNA transport in neurons and its translation. We identified several RNAs associated with FMRP by APRA-microarray. One of them is R1, which is a transcription regulator of Monoamine oxidase-A (MAO-A). Now we are tried to detect the MAO-A activity in wild type and FMRP knockout mice. And the MAO substrate we are using is fluorescent protein- Kynuramine. The technique in LFD is very important for us to get the results.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。FMRP是脆性X综合征的突变蛋白，脆性X综合征是人类常见的一种智力低下。FMRP也是一种RNA结合蛋白，可能调节神经元中的RNA运输及其翻译。我们利用APRA-微阵列技术鉴定了几个与FMRP相关的RNAs。其中一个是R1，它是单胺氧化酶-A(MAO-A)的转录调节因子。现在我们尝试检测野生型和FMRP基因敲除小鼠的MAO-A活性。而我们使用的MAO底物是荧光蛋白-犬尿胺。LFD中的技术对于我们得到结果是非常重要的。