SPINE MORPHOLOGY IN AN ANIMAL MODEL OF FRAGILE X SYNDROME

脆性 X 综合征动物模型的脊柱形态

• 批准号: 7722395
• 负责人: WILLIAM T GREENOUGH
• 金额: $ 3.32万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722395
• 关键词: Animal Model; Appearance; Autopsy; Computer Retrieval of Information on Scientific Projects Database; Dendrites; Dendritic Spines; Fragile X Mental Retardation Protein; Fragile X Syndrome; Funding; Golgi Apparatus; Grant; Human; Institution; Morphology; Research; Research Personnel; Resources; Source; Synapses; Three-Dimensional Imaging; Tissues; United States National Institutes of Health; Vertebral column; mouse model; voltage

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Golgi-impregnation studies of human autopsy tissue and of a mouse model of fragile X mental retardation syndrome suggest that dendritic spines have an underdeveloped appearance--a tendency towards elongated, tortuous spine morphology. We have tentatively hypothesized that the absence of the fragile X protein (FMRP) interferes with synaptic and dendritic maturation. We wish to examine dendritic segments using high voltage EM to provide 3-D images of dendrites and their spines to confirm and provide more details with respect to the maturation hypothesis.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 对人体尸检组织和脆性 X 智力低下综合征小鼠模型的高尔基体浸渍研究表明，树突棘具有不发达的外观，即有拉长、曲折的脊柱形态倾向。我们初步假设脆性 X 蛋白 (FMRP) 的缺失会干扰突触和树突的成熟。我们希望使用高压 EM 检查树突片段，以提供树突及其脊柱的 3D 图像，以确认并提供有关成熟假设的更多细节。