SPINE MORPHOLOGY IN AN ANIMAL MODEL OF FRAGILE X SYNDROME

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 对人类尸检组织和脆性X染色体智力低下综合征小鼠模型的高尔基体浸渍研究表明，树突棘具有不发达的外观-倾向于细长，曲折的棘形态。我们已经初步假设，缺乏脆性X蛋白（FMRP）干扰突触和树突的成熟。我们希望使用高电压EM检查树突节段，以提供树突及其棘的3-D图像，以确认成熟假说并提供更多细节。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Golgi-impregnation studies of human autopsy tissue and of a mouse model of fragile X mental retardation syndrome suggest that dendritic spines have an underdeveloped appearance--a tendency towards elongated, tortuous spine morphology. We have tentatively hypothesized that the absence of the fragile X protein (FMRP) interferes with synaptic and dendritic maturation. We wish to examine dendritic segments using high voltage EM to provide 3-D images of dendrites and their spines to confirm and provide more details with respect to the maturation hypothesis.