LOOKING FOR RNA CARGOS REGULATED BY FMRP

寻找受 FMRP 监管的 RNA 货物

• 批准号: 7600947
• 负责人: WILLIAM T GREENOUGH
• 金额: $ 0.7万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7600947
• 关键词: CT2584 HMS; Computer Retrieval of Information on Scientific Projects Database; FMRP; Fragile X Syndrome; Funding; Genetic Transcription; Grant; Human; Institution; Knockout Mice; Kynuramine; Mental Retardation; Monoamine Oxidase A; Mutate; Neurons; Proteins; RNA Transport; RNA-Binding Proteins; Research; Research Personnel; Resources; Source; Techniques; Translations; United States National Institutes of Health

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. FMRP is the protein mutated from fragile X syndrome, which is a common mental retardation in human. FMRP is also a RNA binding protein and may regulate RNA transport in neurons and its translation. We identified several RNAs associated with FMRP by APRA-microarray. One of them is R1, which is a transcription regulator of Monoamine oxidase-A (MAO-A). Now we are tried to detect the MAO-A activity in wild type and FMRP knockout mice. And the MAO substrate we are using is fluorescent protein- Kynuramine. The technique in LFD is very important for us to get the results.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 FMRP是脆性X综合征突变的蛋白质，脆性X综合征是人类常见的智力低下。FMRP也是一种RNA结合蛋白，可以调节神经元中的RNA转运及其翻译。我们通过APRA-微阵列鉴定了几种与FMRP相关的RNA。其中之一是R1，它是单胺氧化酶-A（MAO-A）的转录调节因子。现在我们试图检测野生型和FMRP基因敲除小鼠中MAO-A的活性。我们使用的微弧氧化底物是荧光蛋白-犬尿胺。LFD中的技术对于我们得到结果是非常重要的。