T1-RELAXIVITIES AS FUNCTION OF PORPHYRINS AND TEMPERATURE

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This aim of this project is to develop a cisplatin/gadoteridol temperature-sensitive liposome (TSL) that will allow the use of MRI T1 shortening to measure the liposomal concentration of cisplatin. A secondary goal of this work is to evaluate the relaxivity of several manganese porphyrins, and test the potential of their encapsulation into TSL. The project first requires calibration of 1/T1 as a function of free gadoteridol concentration at room temperature in the presence of lipid. This calibration will be used to verify liposomal loading and release in vitro. In the final liposome formulation, gadoteridol and cisplatin will both be encapsulated into the liposomes, and T1 relaxivity will be measured before and after liposome release. To prepare for future in vivo studies in combination with hyperthermia, it is also necessary to calibrate T1 relaxivity as a function of temperature. The same procedure will be applied to in vitro tests that involve manganese porphyrin solutions, with the purpose of determination of their relaxivity, and release characteristics of TSL liposomes that will be used to encapsulate them.