Correlation of metabolic fingerprints with differential growth responses of cold-inhibited salicylate mutants

代谢指纹与冷抑制水杨酸盐突变体差异生长反应的相关性

• 批准号: BB/D006651/1
• 负责人: Ian Scott
• 金额: $ 1.65万
• 依托单位: Aberystwyth University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FD006651%2F1
• 关键词: Correlation; metabolic; fingerprints; differential; growth

Cold is a major environmental stress that determines the agricultural and horticultural productivity of particular geographical locations. Low temperatures impact meat as well as crop production, by limiting early-season growth of forage grass. Climate change adds a major uncertainty to future projections of world food production, with some current theories indicating cooling rather than warming of the UK climate. Last year we published a new discovery on the hormonal controls of plant growth at low temperature, finding mutants of the model plant Arabidopsis thaliana that showed much stronger than normal growth in controlled environments cooled to 5oC. This research was financed by the BBSRC Plant and Microbial Sciences Committee [Scott IM, Clarke SM, Wood JE, Mur LAJ (2004) Salicylate accumulation inhibits growth at chilling temperature in Arabidopsis. Plant Physiology 135:1040-1049]. The present proposal is a follow-up, which capitalises on the launch of the BBSRC's Plant and Microbial Metabolomics (MeT-RO) Centre, and seeks to use these facilities to analyse a wide spectrum of the low molecular weight biochemicals in plant cells, to produce so-called 'metabolic fingerprints'. Our preliminary studies show that metabolic fingerprints can be used to classify Arabidopsis mutants according to their propensity for growth at low temperature. The MeT-RO Centre service will produce detailed and informative metabolic fingerprints from which we will learn a great deal about the cellular states that determine the ability of plants to grow more strongly in the cold.

寒冷是一种主要的环境压力，它决定了特定地理位置的农业和园艺生产力。低温通过限制牧草的早期生长来影响肉类和作物生产。气候变化给未来世界粮食产量的预测增加了一个主要的不确定性，目前一些理论表明英国的气候会变冷而不是变暖。去年，我们发表了一项关于激素控制植物在低温下生长的新发现，发现模式植物拟南芥（Arabidopsis thaliana）的突变体在温度降至5摄氏度的受控环境下比正常生长强得多。本研究由BBSRC植物与微生物科学委员会资助[Scott IM， Clarke SM, Wood JE, Mur LAJ(2004)]。水杨酸盐积累抑制拟南芥在低温下的生长。植物生理学[j]。目前的提案是一个后续，它利用BBSRC的植物和微生物代谢组学（MeT-RO）中心的启动，并寻求使用这些设施来分析植物细胞中低分子量生化物质的广泛光谱，以产生所谓的“代谢指纹”。我们的初步研究表明，代谢指纹图谱可以根据拟南芥在低温下的生长倾向对其进行分类。MeT-RO中心的服务将产生详细和信息丰富的代谢指纹，从中我们将了解很多关于细胞状态的信息，这些状态决定了植物在寒冷中生长得更强的能力。

项目成果

Opposite variations in fumarate and malate dominate metabolic phenotypes of Arabidopsis salicylate mutants with abnormal biomass under chilling.

• DOI: 10.1111/ppl.12210
• 发表时间: 2014-12
• 期刊: Physiologia plantarum
• 影响因子: 6.4
• 作者: I. Scott;J. Ward;Sonia J. Miller;M. H. Beale