ANIONIC LIPIDS AND MITOCHONDRIAL STRUCTURE

阴离子脂质和线粒体结构

• 批准号: 7358111
• 负责人: WILLIAM DOWHAN
• 金额: $ 0.2万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358111
• 关键词: ANIONIC; LIPIDS; MITOCHONDRIAL; STRUCTURE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dowhan's group has isolated mutants of Saccharomyces cerevisiae that lack the mitochondrial specific lipids cardiolipin and phosphatidylglycerol (pgs1) or just lack cardiolipin with increased phosphatidylglycerol (crd1). Pgs1 cells are respiratory deficient, have low mitochondrial membrane potential,and are blocked in the translation of all mitochondrial encoded components of the respiratory chain. They are viable on fermentable carbon sources and we know by light microscopy that their mitochondrial morphology is altered. Crd1 cells grow reasonably well by respiration but we know that the supermolecular complex normally present between Complexes III and IV of the respiratory chain is destabilized in the absense of cardiolipin. This strain also shows grow defects when stressed under respiratory growth conditions. They are interested in detailed morphological information on the structure of the mitochondria of both cells types. In particular tomagraphic reconstruction of mitochondria and their cristae is of interest. If organization of complexes along the cristae is possible, this would also be of interest to correlate with our biochemical data. At the moment they would rely on the NCMIR personel to carry out the studies -- although given protocols by NCMIR, samples would be prepared in Houston.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。Dohhan的研究小组已经分离出酿酒酵母的突变体，这些突变体缺乏线粒体特异性脂质心磷脂和磷脂酰甘油（pgs 1），或者只是缺乏心磷脂并增加了磷脂酰甘油（crd 1）。pgs 1细胞是呼吸缺陷的，具有低线粒体膜电位，并且在呼吸链的所有线粒体编码组分的翻译中被阻断。它们在可发酵的碳源上是可行的，我们通过光学显微镜知道它们的线粒体形态发生了改变。Crd 1细胞通过呼吸生长得相当好，但我们知道，在心磷脂缺乏的情况下，呼吸链的复合物III和IV之间通常存在的超分子复合物是不稳定的。该菌株在呼吸生长条件下应激时也显示出生长缺陷。他们对这两种细胞类型线粒体结构的详细形态学信息感兴趣。特别是线粒体和它们的嵴的断层摄影重建是感兴趣的。如果复合物沿着嵴的组织是可能的，这也将是与我们的生物化学数据相关联的兴趣。目前，他们将依靠NCIR的人员进行研究-尽管NCIR规定了方案，但样品将在休斯顿制备。