ANIONIC LIPIDS AND MITOCHONDRIAL STRUCTURE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。杜哈恩的团队已经分离出缺乏线粒体特异性脂质心磷脂和磷脂酰甘油(Pgs1)的酿酒酵母突变株，或者只是缺乏心磷脂而磷脂酰甘油(Crd1)增加的突变株。Pgs1细胞呼吸障碍，线粒体膜电位低，呼吸链所有线粒体编码成分的翻译受阻。它们在可发酵的碳源上是可行的，我们通过光学显微镜知道它们的线粒体形态发生了变化。Crd1细胞通过呼吸作用生长得相当好，但我们知道，通常存在于呼吸链复合体III和IV之间的超分子复合体在没有心磷脂的情况下是不稳定的。这种菌株在呼吸生长条件下受到压力时也显示出生长缺陷。他们对这两种细胞线粒体结构的详细形态信息感兴趣。尤其是线粒体及其脊的断层重建是很有意义的。如果可能沿着眉骨组织复合体，这也将与我们的生化数据相关联。目前，他们将依靠NCMIR的人员进行研究--尽管NCMIR提供了协议，但样本将在休斯顿准备。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dowhan's group has isolated mutants of Saccharomyces cerevisiae that lack the mitochondrial specific lipids cardiolipin and phosphatidylglycerol (pgs1) or just lack cardiolipin with increased phosphatidylglycerol (crd1). Pgs1 cells are respiratory deficient, have low mitochondrial membrane potential,and are blocked in the translation of all mitochondrial encoded components of the respiratory chain. They are viable on fermentable carbon sources and we know by light microscopy that their mitochondrial morphology is altered. Crd1 cells grow reasonably well by respiration but we know that the supermolecular complex normally present between Complexes III and IV of the respiratory chain is destabilized in the absense of cardiolipin. This strain also shows grow defects when stressed under respiratory growth conditions. They are interested in detailed morphological information on the structure of the mitochondria of both cells types. In particular tomagraphic reconstruction of mitochondria and their cristae is of interest. If organization of complexes along the cristae is possible, this would also be of interest to correlate with our biochemical data. At the moment they would rely on the NCMIR personel to carry out the studies -- although given protocols by NCMIR, samples would be prepared in Houston.