ANIONIC LIPIDS AND MITOCHONDRIAL STRUCTURE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。Dowhan的研究小组已经分离出了酿酒酵母的突变体，这些突变体缺乏线粒体特异性脂质心肌磷脂和磷脂酰甘油（pgs1），或者只是缺乏心肌磷脂而增加了磷脂酰甘油（crd1）。Pgs1细胞呼吸缺陷，线粒体膜电位低，呼吸链中所有线粒体编码成分的翻译被阻断。它们在可发酵的碳源上存活，我们通过光学显微镜知道它们的线粒体形态被改变了。Crd1细胞通过呼吸生长得相当好，但我们知道，通常存在于呼吸链复合物III和IV之间的超分子复合物在缺乏心磷脂的情况下是不稳定的。该菌株在呼吸生长条件下也表现出生长缺陷。他们对这两种细胞线粒体结构的详细形态学信息很感兴趣。特别是线粒体及其嵴的断层重建是感兴趣的。如果沿嵴组织复合体是可能的，这也将与我们的生化数据相关。目前，他们将依靠NCMIR人员进行研究——尽管NCMIR提供了协议，但样本将在休斯顿制备。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dowhan's group has isolated mutants of Saccharomyces cerevisiae that lack the mitochondrial specific lipids cardiolipin and phosphatidylglycerol (pgs1) or just lack cardiolipin with increased phosphatidylglycerol (crd1). Pgs1 cells are respiratory deficient, have low mitochondrial membrane potential,and are blocked in the translation of all mitochondrial encoded components of the respiratory chain. They are viable on fermentable carbon sources and we know by light microscopy that their mitochondrial morphology is altered. Crd1 cells grow reasonably well by respiration but we know that the supermolecular complex normally present between Complexes III and IV of the respiratory chain is destabilized in the absense of cardiolipin. This strain also shows grow defects when stressed under respiratory growth conditions. They are interested in detailed morphological information on the structure of the mitochondria of both cells types. In particular tomagraphic reconstruction of mitochondria and their cristae is of interest. If organization of complexes along the cristae is possible, this would also be of interest to correlate with our biochemical data. At the moment they would rely on the NCMIR personel to carry out the studies -- although given protocols by NCMIR, samples would be prepared in Houston.