MACCHESS PROGRAM FOR MICROCRYSTALLOGRAPHY/MEMBRANE PROTEIN CRYSTALS

微晶学/膜蛋白晶体的 MACCHESS 程序

• 批准号: 7357710
• 负责人: MARTIN D. CAFFREY
• 金额: $ 5.67万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357710
• 关键词: MACCHESS; PROGRAM; MICROCRYSTALLOGRAPHY; MEMBRANE; PROTEIN

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have just begun diffraction studies (F1 line) on the membrane proteins grown in-house by the in meso method. Measurements were made during our first trip to CHESS under the auspices of this grant in September, 2003. Data were collected using cryocooled microcrystals of maximum dimension 40 micron. Best results to date are diffraction to 1.8 ¿ for bacteriorhodopsin (bR) and 2.8 ¿ for the light harvesting complex II (Lh2) protein. Structures of bR have been solved using molecular replacement. Diffraction data statistics for bR/Lh2 are as follows: Space group: P63 / P212121; Resolution limits (¿): 40 - 1.85/50 - 2.8; Unique reflections: 20,214/26,790; Completeness (%): 96.7/98.6; I/sigma: 29.9/13.3; Rsym (%): 7.0/9.4.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们刚刚开始衍射研究（F1线）的膜蛋白生长在内部的中方法。测量是在我们的第一次国际象棋之旅的赞助下，在2003年9月。使用最大尺寸为40微米的低温冷却微晶收集数据。迄今为止，最好的结果是细菌视紫红质（bR）衍射到1.8，捕光复合物II（Lh 2）蛋白衍射到2.8。bR的结构已解决了使用分子置换。bR/Lh 2的衍射数据统计如下：空间群：P63 / P212121;分辨率极限（<$）：40 - 1.85/50 - 2.8;独特反射：20，214/26，790;完整性（%）：96.7/98.6; I/sigma：29.9/13.3; Rsym（%）：7.0/9.4。