MACCHESS PROGRAM FOR MICROCRYSTALLOGRAPHY/MEMBRANE PROTEIN CRYSTALS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们刚刚开始对用In Meso方法培养的膜蛋白进行衍射研究(F1线)。测量是在2003年9月我们在这笔赠款下的第一次国际象棋之旅中进行的。使用最大尺寸为40微米的冷冻冷却微晶体收集数据。到目前为止，最好的结果是细菌视紫红质(BR)的衍射率为1.8？，捕光复合体II(LH2)的衍射率为2.8？用分子取代法解决了BR的结构问题。BR/LH2的衍射数据统计如下：空间群：P63/P212121；分辨率极限(？)：40-1.85/50-2.8；独特反射：20,214/26,790；完整性(%)：96.7/98.6；I/sigma：29.9/13.3；Rsym(%)：7.0/9.4。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have just begun diffraction studies (F1 line) on the membrane proteins grown in-house by the in meso method. Measurements were made during our first trip to CHESS under the auspices of this grant in September, 2003. Data were collected using cryocooled microcrystals of maximum dimension 40 micron. Best results to date are diffraction to 1.8 ¿ for bacteriorhodopsin (bR) and 2.8 ¿ for the light harvesting complex II (Lh2) protein. Structures of bR have been solved using molecular replacement. Diffraction data statistics for bR/Lh2 are as follows: Space group: P63 / P212121; Resolution limits (¿): 40 - 1.85/50 - 2.8; Unique reflections: 20,214/26,790; Completeness (%): 96.7/98.6; I/sigma: 29.9/13.3; Rsym (%): 7.0/9.4.