STUDIES OF MEMBRANE PROTEINS USING MICROCRYSTALS GROWN IN LIPIDIC MESOPHASES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 为了理解膜蛋白的功能，解决其结构仍然是整个生物学的重大挑战之一。鉴于大多数基因组中至少有四分之一编码膜蛋白，其中许多是疾病病原体和治疗剂的目标，迎接挑战的健康后果是巨大的。我们采取多方面的方法，通过生产用于大分子晶体学的晶体来促进我们对膜蛋白功能的理解。通过内消旋方法在非晶中间相中结晶正用于真核生物和原核生物、整体和外周、单体和多聚体蛋白质，以及蛋白质-蛋白质和蛋白质-肽复合物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Solving the structure with a view to understanding the function of membrane proteins remains one of the grand challenges in all of Biology. Given that at least a quarter of most genomes codes for membrane proteins, many of which are targets of disease causative and treatment agents, the health consequences of meeting the challenge are great. We take a multifaceted approach to advancing our understanding of membrane protein function by producing crystals for use in macromolecular crystallography. Crystallization in lipidic mesophases by the in meso method is being used with eukaryote and prokaryote, integral and peripheral, monomeric and multimeric proteins, as well as with protein-protein and protein-peptide complexes.