STUDIES OF MEMBRANE PROTEINS USING MICROCRYSTALS GROWN IN LIPIDIC MESOPHASES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 解决结构以了解膜蛋白的功能仍然是整个生物学的重大挑战之一。鉴于至少四分之一的大多数基因组编码膜蛋白，其中许多是致病和治疗药物的靶标，迎接挑战的健康后果是巨大的。我们采取多方面的方法，通过生产用于大分子结晶学的晶体来提高我们对膜蛋白功能的理解。用In介观方法在脂类中间相结晶的方法用于真核生物和原核生物、整体和外围、单体和多聚体蛋白质，以及蛋白质-蛋白质和蛋白质-肽复合体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Solving the structure with a view to understanding the function of membrane proteins remains one of the grand challenges in all of Biology. Given that at least a quarter of most genomes codes for membrane proteins, many of which are targets of disease causative and treatment agents, the health consequences of meeting the challenge are great. We take a multifaceted approach to advancing our understanding of membrane protein function by producing crystals for use in macromolecular crystallography. Crystallization in lipidic mesophases by the in meso method is being used with eukaryote and prokaryote, integral and peripheral, monomeric and multimeric proteins, as well as with protein-protein and protein-peptide complexes.