CONTROL OF MITOCHONDRIAL FUNCTION BY NITRIC OXIDE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。Heck博士对紫外光诱导的氧化应激机制作为致癌机制感兴趣。她一直在描述内源性细胞光敏剂和抗氧化剂，它们在保护细胞免受紫外线介导的毒性方面形成平衡。赫克博士发现，一氧化氮是紫外线作用的关键媒介。她的研究重点是确定一氧化氮是抗氧化剂还是促氧化剂。为此，她一直活跃在BRC开发和验证一氧化氮自我参考生物传感器。在BRC中，她描述了各种细胞类型产生一氧化氮的特征。她也一直在研究过氧亚硝酸盐在介导细胞氧化应激中的作用，使用自参考氧生物传感器进行测量。在与Joshua Gray的合作中，她一直在研究高氧在介导细胞一氧化氮生成中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dr. Heck is interested in mechanisms of oxidative stress induced by ultraviolet light as a mechanism of carcinogenesis. She has been characterizing endogenous cellular photosensitizers and antioxidants that form a balance in protecting cells from ultraviolet light-mediated toxicity. Dr. Heck has found that nitric oxide is a key mediator of the actions of ultraviolet light. Her research has focused on determining if nitric oxide functions as an anti-oxidant of a pro-oxidant. Towards this end she has been active in the BRC in developing and validating a nitric oxide self-referencing biosensor. In the BRC she has been characterizing nitric oxide production by a variety of cell types. She has also been investigating the role of peroxynitrite in mediating cellular oxidative stress as measured using a self-referencing oxygen biosensor. In collaboration with Joshua Gray, she has been investigating the role of hyperoxia in mediating cellular nitric oxide production.