CHEMICAL AND ENVIRONMENTAL EFFECTS ON MAMMALIAN SKIN

化学和环境对哺乳动物皮肤的影响

• 批准号: 7357368
• 负责人: Michael Peter Shakarjian
• 金额: $ 1.23万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357368
• 关键词: CHEMICAL; ENVIRONMENTAL; EFFECTS; MAMMALIAN; SKIN

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our current studies at the BRC involve the physiological function of the epidermal layer of mammalian skin and its response to various chemical and environmental stimuli. The mammalian epidermis is a multilayered organ, serving to control water loss and protect the organism from external toxins and pathogens. The maintenance of the skin¿s integrity is essential for health and survival of the organism, and loss of epidermal barrier function has been implicated in responses to chemical insult as well as a number of disorders, including psoriasis and atopic dermatitis. In the skin, basal keratinocytes, self-renewing cells located at the base of the epidermis, grow, differentiate, and give rise to the cell layers above. In this process an extracellular pH gradient is formed, in which the outermost differentiated layers are most acidic. Acidification is driven by the epidermal cell proton extrusion, creating an environment essential for barrier integrity. We were interested in examining the mechanisms involved in extracellular acidification by keratinocytes, and recognized that NCRR BioCurrents Research Center had resources that were uniquely suited to monitoring extracellular pH from these cells.

该主题项目是利用NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是针对该中心的，这不是调查人员的机构。我们目前在BRC的研究涉及哺乳动物皮肤表皮层的生理功能及其对各种化学和环境刺激的反应。哺乳动物表皮是一种多层器官，可控制水分流失并保护有机体免受外部毒素和病原体的侵害。皮肤的维持对生物体的健康和生存至关重要，并且在对化学侮辱以及多种疾病的反应中已经实施了表皮屏障功能的丧失，包括牛皮癣和特征性皮炎。在皮肤中，碱性角质形成细胞，位于表皮底部的自我更新细胞，生长，分化并产生上面的细胞层。在此过程中，形成了细胞外pH梯度，其中最外面的分化层是最酸性的。酸化是由表皮细胞质子扩展驱动的，从而为屏障完整性带来了必不可少的环境。我们有兴趣检查角质形成细胞涉及细胞外酸化的机制，并认识到NCRR Biocurrents研究中心的资源非常适合从这些细胞中监测细胞外pH。