FUNCTIONAL HETEROGENEITY OF MITOCHONDRIA IN AN INDIVIDUAL PANCREATIC BETA CELL

单个胰腺β细胞中线粒体的功能异质性

• 批准号: 7357321
• 负责人: Orian S Shirihai
• 金额: $ 0.61万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357321
• 关键词: FUNCTIONAL; HETEROGENEITY; MITOCHONDRIA; INDIVIDUAL; PANCREATIC

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Communication of mitochondria through networking supports fuel metabolism and calcium transport, and impacts the cellular response to apoptotic stimuli. A mitochondrial network shares a continuous matrix lumen that enables the diffusion of molecules and allows for electrical coupling and synchronization. However, the identification of a network and distinguishing it from closely packed but disconnected mitochondria remains a challenge, and thus it is unclear how common and how dynamic functional networks are. We used the resources at the BioCurrent Research Center tissue culture, confocal microscopy and chemical databases and fluorescent probes to set up a methodology to functionally define the network in time and space and study its electrical and diffusion properties overtime.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。线粒体通过网络进行的通讯支持燃料代谢和钙运输，并影响细胞对细胞凋亡刺激的反应。线粒体网络共享一个连续的基质腔，可以实现分子的扩散并允许电耦合和同步。然而，识别网络并将其与紧密堆积但断开连接的线粒体区分开来仍然是一个挑战，因此尚不清楚功能网络有多常见和动态如何。我们利用 BioCurrent 研究中心的组织培养资源、共焦显微镜、化学数据库和荧光探针建立了一种方法，可以在时间和空间上功能性地定义网络，并随着时间的推移研究其电学和扩散特性。