FUNCTIONAL HETEROGENEITY OF MITOCHONDRIA IN AN INDIVIDUAL PANCREATIC BETA CELL

单个胰腺β细胞中线粒体的功能异质性

• 批准号: 7357321
• 负责人: Orian S Shirihai
• 金额: $ 0.61万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357321
• 关键词: FUNCTIONAL; HETEROGENEITY; MITOCHONDRIA; INDIVIDUAL; PANCREATIC

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Communication of mitochondria through networking supports fuel metabolism and calcium transport, and impacts the cellular response to apoptotic stimuli. A mitochondrial network shares a continuous matrix lumen that enables the diffusion of molecules and allows for electrical coupling and synchronization. However, the identification of a network and distinguishing it from closely packed but disconnected mitochondria remains a challenge, and thus it is unclear how common and how dynamic functional networks are. We used the resources at the BioCurrent Research Center tissue culture, confocal microscopy and chemical databases and fluorescent probes to set up a methodology to functionally define the network in time and space and study its electrical and diffusion properties overtime.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。线粒体通过网络的通信支持燃料代谢和钙转运，并影响细胞对凋亡刺激的反应。线粒体网络共享一个连续的矩阵腔，可实现分子的扩散并允许电耦合和同步。但是，识别网络并将其与密切包装但断开的线粒体区分开来仍然是一个挑战，因此目前尚不清楚动态功能网络的共同点和程度。我们在BioCurrent研究中心组织培养，共聚焦显微镜和化学数据库和荧光探针上使用了资源来建立一种方法，以在时间和空间中定义网络并研究其电气和扩散性能的加时性。