FUNCTIONAL HETEROGENEITY OF MITOCHONDRIA IN AN INDIVIDUAL PANCREATIC BETA CELL

点击翻译按钮获取中文摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Communication of mitochondria through networking supports fuel metabolism and calcium transport, and impacts the cellular response to apoptotic stimuli. A mitochondrial network shares a continuous matrix lumen that enables the diffusion of molecules and allows for electrical coupling and synchronization. However, the identification of a network and distinguishing it from closely packed but disconnected mitochondria remains a challenge, and thus it is unclear how common and how dynamic functional networks are. We used the resources at the BioCurrent Research Center tissue culture, confocal microscopy and chemical databases and fluorescent probes to set up a methodology to functionally define the network in time and space and study its electrical and diffusion properties overtime.