FUNCTIONAL HETEROGENEITY OF MITOCHONDRIA IN AN INDIVIDUAL PANCREATIC BETA CELL

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。线粒体通过网络的通讯支持燃料代谢和钙的运输，并影响细胞对凋亡刺激的反应。线粒体网络共享一个连续的基质管腔，使分子能够扩散，并允许电耦合和同步。然而，识别网络并将其与紧密排列但互不相连的线粒体区分开来仍然是一个挑战，因此尚不清楚功能网络的普遍程度和动态程度。我们利用BioCurrent研究中心的组织培养、共聚焦显微镜和化学数据库以及荧光探针的资源，建立了一种方法来在时间和空间上对网络进行功能定义，并长期研究其电学和扩散特性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Communication of mitochondria through networking supports fuel metabolism and calcium transport, and impacts the cellular response to apoptotic stimuli. A mitochondrial network shares a continuous matrix lumen that enables the diffusion of molecules and allows for electrical coupling and synchronization. However, the identification of a network and distinguishing it from closely packed but disconnected mitochondria remains a challenge, and thus it is unclear how common and how dynamic functional networks are. We used the resources at the BioCurrent Research Center tissue culture, confocal microscopy and chemical databases and fluorescent probes to set up a methodology to functionally define the network in time and space and study its electrical and diffusion properties overtime.