FUNCTIONAL HETEROGENEITY OF MITOCHONDRIA IN AN INDIVIDUAL PANCREATIC BETA CELL

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。线粒体通过网络的通信支持燃料代谢和钙转运，并影响细胞对凋亡刺激的反应。线粒体网络共享连续的基质腔，其使得分子能够扩散并允许电耦合和同步。然而，识别网络并将其与紧密包装但断开的线粒体区分开来仍然是一个挑战，因此尚不清楚网络的常见程度和动态功能。我们利用生物电流研究中心组织培养、共聚焦显微镜和化学数据库以及荧光探针的资源，建立了一种方法来在时间和空间上功能性地定义网络，并研究其随时间推移的电学和扩散特性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Communication of mitochondria through networking supports fuel metabolism and calcium transport, and impacts the cellular response to apoptotic stimuli. A mitochondrial network shares a continuous matrix lumen that enables the diffusion of molecules and allows for electrical coupling and synchronization. However, the identification of a network and distinguishing it from closely packed but disconnected mitochondria remains a challenge, and thus it is unclear how common and how dynamic functional networks are. We used the resources at the BioCurrent Research Center tissue culture, confocal microscopy and chemical databases and fluorescent probes to set up a methodology to functionally define the network in time and space and study its electrical and diffusion properties overtime.