MITOCHONDRIAL RESPIROMETRY IN FROZEN BIOLOGICAL SAMPLES
冷冻生物样品中的线粒体呼吸测定
基本信息
- 批准号:10011475
- 负责人:
- 金额:$ 22.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-05-20 至 2021-04-30
- 项目状态:已结题
- 来源:
- 关键词:AcademiaAcuteAgingBiocompatible MaterialsBiologicalBiological AssayBiotechnologyCardiovascular systemCellsClientClinicalClinical ResearchCommunitiesComplexDataDevelopmentDiseaseDrug ExposureEconomicsEnvironmentEnvironmental MonitoringEvaluationFeedbackFreezingFutureGenetic ModelsGoalsHourHumanImpairmentIndustryInstructionLegal patentMeasurementMeasuresMetabolicMethodologyMethodsMitochondriaMitochondrial DiseasesMonitorMusOrganizational ModelsOutcomeOxygen ConsumptionPatientsPharmaceutical PreparationsPharmacologic SubstancePhasePlayPopulationPositioning AttributePreparationProceduresProtocols documentationReagentReproducibilityResearchResearch ContractsResearch PersonnelRespirationRespiratory physiologyRunningSamplingServicesShipsSideSiteSwabTechnologyTechnology AssessmentTestingTimeTissue SampleTissuesToxicant exposureToxicologyUnited States Food and Drug Administrationclinical applicationclinical developmentclinical practiceclinically relevantenvironmental toxicologyimprovedminimally invasivenew technologyrespiratorytoxicant
项目摘要
Abstract
Impaired mitochondrial respiration plays a key role in metabolic, cardiovascular, and aging-related
diseases. However, mitochondrial respirometry analysis currently requires processing of the living tissue
sample within an hour after being taken from the patient. This requirement makes respirometry analysis largely
unfeasible to standard clinical practice and clinical studies. We invented a new technology to assess maximal
mitochondrial respiratory capacity in previously-frozen biological samples, which thus far been considered
impossible by the scientific community. Side-by-side comparison confirmed that our assay accurately reflects
results measured in fresh samples. Our technology thus overcomes the fundamental limitation of standard
approaches that required living tissue. Moreover, our approach consists of simplified sample preparation which
uses significantly less biological material.
Enspire Bio will further develop this patented Frozen Mitos technology to enable respirometry
measurements for clinical applications and frozen samples, which currently is not available. We will achieve
our goals by Aim 1: Establishing standard protocols for oxygen consumption measurements from frozen tissue
samples derived from human and mouse tissue samples. We will further improve throughput by enabling
measurements of oxygen consumption from tissue homogenate instead of isolated mitochondria. Aim 2:
Establishing proprietary kits and delivering those to five beta testing sites including opinion-leading academic
labs, mitochondrial disease centers and a biotech and pharmaceutical company to test and provide feedback.
To provide frozen sample analysis to clients that have no capacity for wet lab assays, we will develop a CRO-
type service in-house where samples will be shipped to EnspireBio for analysis. Working with a set of pilot
academic, clinical and industry clients will provide feedback to establish procedures and the economics of the
CRO model approach for the frozen tissue respirometry.
摘要
线粒体呼吸受损在代谢、心血管和衰老相关疾病中起着关键作用
疾病。然而,线粒体呼吸测量分析目前需要对活组织进行处理
在从病人身上取下样本后一小时内。这一要求使得呼吸测量分析变得非常重要
对于标准的临床实践和临床研究是不可行的。我们发明了一种新技术来评估最大
之前冷冻的生物样本中的线粒体呼吸能力,到目前为止还被认为是
科学界认为这是不可能的。并列比对证实了我们的分析准确地反映了
结果在新鲜样品中测量。因此,我们的技术克服了标准的根本限制
需要活组织的方法。此外,我们的方法包括简化的样品制备,该方法
使用的生物材料显著减少。
Enspire Bio将进一步开发这项获得专利的冷冻Mitos技术,以实现呼吸测量
临床应用和冷冻样本的测量,目前无法获得。我们将实现
我们的目标1:建立冰冻组织耗氧量测量的标准方案
从人类和小鼠组织样本中提取的样本。我们将通过以下方式进一步提高吞吐量
测量组织匀浆的耗氧量,而不是分离的线粒体。目标2:
建立专有套件并将其交付给五个Beta测试站点,其中包括意见领先的学术网站
实验室、线粒体疾病中心以及一家生物技术和制药公司进行测试并提供反馈。
为了向没有能力进行湿法实验室分析的客户提供冷冻样本分析,我们将开发一种CRO-
在内部服务类型中,样品将被运送到EnspirreBio进行分析。与一组飞行员一起工作
学术、临床和行业客户将提供反馈,以制定程序和经济
冰冻组织呼吸测量的CRO模型方法。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Orian S Shirihai其他文献
Cardiac-Specific Fatty Acid Transport Protein 1 (FATP1) Overexpression Causes Decreased Mitochondrial Respiration, Increased Oxidative Stress and Activation of AMPK
- DOI:
10.1016/j.freeradbiomed.2012.10.434 - 发表时间:
2012-11-01 - 期刊:
- 影响因子:
- 作者:
Aly Elezaby;Edward J Miller;Fuzhong Qin;Marc Liesa;Orian S Shirihai;Wilson S Colucci - 通讯作者:
Wilson S Colucci
Orian S Shirihai的其他文献
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{{ truncateString('Orian S Shirihai', 18)}}的其他基金
MITOCHONDRIAL RESPIROMETRY IN FROZEN BIOLOGICAL SAMPLES
冷冻生物样品中的线粒体呼吸测定
- 批准号:
10251412 - 财政年份:2021
- 资助金额:
$ 22.5万 - 项目类别:
Role of the heme-related mitochondrial antioxidant ABCB10 in alcoholic liver disease
血红素相关线粒体抗氧化剂 ABCB10 在酒精性肝病中的作用
- 批准号:
10201420 - 财政年份:2019
- 资助金额:
$ 22.5万 - 项目类别:
Role of the heme-related mitochondrial antioxidant ABCB10 in alcoholic liver disease
血红素相关线粒体抗氧化剂 ABCB10 在酒精性肝病中的作用
- 批准号:
10443585 - 财政年份:2019
- 资助金额:
$ 22.5万 - 项目类别:
Mitochondrial dynamics in beta cell function and dysfunction
β细胞功能和功能障碍的线粒体动力学
- 批准号:
7645363 - 财政年份:2007
- 资助金额:
$ 22.5万 - 项目类别:
Mitochondrial dynamics in beta cell function and dysfunction
β细胞功能和功能障碍的线粒体动力学
- 批准号:
8012816 - 财政年份:2007
- 资助金额:
$ 22.5万 - 项目类别:
Mitochondrial dynamics in beta cell function and dysfunction
β细胞功能和功能障碍的线粒体动力学
- 批准号:
7346916 - 财政年份:2007
- 资助金额:
$ 22.5万 - 项目类别:
Mitochondrial dynamics in beta cell function and dysfunction
β细胞功能和功能障碍的线粒体动力学
- 批准号:
7535553 - 财政年份:2007
- 资助金额:
$ 22.5万 - 项目类别:
Mitochondrial dynamics in beta cell function and dysfunction
β细胞功能和功能障碍的线粒体动力学
- 批准号:
7212876 - 财政年份:2007
- 资助金额:
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FUNCTIONAL HETEROGENEITY OF MITOCHONDRIA IN AN INDIVIDUAL PANCREATIC BETA CELL
单个胰腺β细胞中线粒体的功能异质性
- 批准号:
7357321 - 财政年份:2005
- 资助金额:
$ 22.5万 - 项目类别:
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