SSK2 ASSOCIATED PROTEINS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。MEK激酶Ssk 2 p是人MEK激酶MTK 1/MEKK 4的同源物，对于这类激酶具有独特的性质，能够调节肌动蛋白细胞骨架以响应渗透胁迫。我们的工作主要集中在酵母中，使用酵母激酶Ssk 2 p，但我们以前已经证明人类激酶具有这些独特的功能。我们从遗传实验中知道，这种激酶的调节独立于其正常的MAPK途径，它直接结合肌动蛋白，在所有酵母MEK激酶中，它具有调节肌动蛋白组装的独特能力。这项合作旨在尝试和鉴定Ssk 2 p相关蛋白，这些蛋白可能负责途径独立的激酶调节，并且可能是激酶的细胞骨架效应子。该方法是TAP标记Ssk 2 p，从酵母中沉淀复合物，并使用质谱鉴定这些相关蛋白。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The MEK kinase Ssk2p, a homolog of the human MEK kinase MTK1/MEKK4 has unique properties for this class of kinases in being able to regulate the actin cytoskeleton in response to osmotic stress. Our work is focused in yeast, with the yeast kinase Ssk2p, but we have previously shown that the human kinase has these unique capabilities. We know from genetic experiments that this kinase is regulated independently of its normal MAPK pathway, that it binds actin directly and among all yeast MEK kinases has this unique capacity to regulate actin assembly. This collaboration was initiated to try and identify Ssk2p-associated proteins that could be responsible for pathway independent kinase regulation and that could be the cytoskeletal effector(s) of the kinase. The approach is to TAP-tag Ssk2p, precipitate complexes from yeast and use mass-spec to identify these associated proteins.