TWO-HYBRID ANALYSIS OF SIFA

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. SifA is a Salmonella effector protein required for the formation of Salmonella-induced filaments that is translocated across the vacuolar membrane enclosing the pathogen and is required for its maintenance. Preliminary data from Miller lab revealed a distinct subcellular localization when SifA was expressed in yeast. The goal of this collaboration is to identify potential yeast proteins that might be binding to SifA and controlling its localization. In turn, this will help elucidate the functional pathway SifA might be involved in within the yeast cell and eventually, used for designing experiments in mammalian cells.