TWO-HYBRID ANALYSIS OF SIFA

SIFA 的两种混合分析

• 批准号: 7420795
• 负责人: DANI B VINH
• 金额: $ 0.5万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420795
• 关键词: Salmonella; fungal proteins; proteins; yeasts

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. SifA is a Salmonella effector protein required for the formation of Salmonella-induced filaments that is translocated across the vacuolar membrane enclosing the pathogen and is required for its maintenance. Preliminary data from Miller lab revealed a distinct subcellular localization when SifA was expressed in yeast. The goal of this collaboration is to identify potential yeast proteins that might be binding to SifA and controlling its localization. In turn, this will help elucidate the functional pathway SifA might be involved in within the yeast cell and eventually, used for designing experiments in mammalian cells.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。 SifA 是沙门氏菌诱导的细丝形成所需的沙门氏菌效应蛋白，该细丝易位穿过包围病原体的液泡膜，并且是其维持所必需的。 Miller 实验室的初步数据显示，当 SifA 在酵母中表达时，存在明显的亚细胞定位。此次合作的目标是鉴定可能与 SifA 结合并控制其定位的潜在酵母蛋白。反过来，这将有助于阐明 SifA 可能参与酵母细胞内的功能途径，并最终用于设计哺乳动物细胞实验。