TWO-HYBRID ANALYSIS OF SIFA

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。SifA是一种沙门氏菌效应蛋白，是沙门氏菌诱导的细丝形成所必需的，该细丝在包裹病原体的空泡膜上易位，并对其维持所必需的。Miller实验室的初步数据显示，SifA在酵母中表达时具有明显的亚细胞定位。这项合作的目标是鉴定可能与SifA结合并控制其定位的潜在酵母蛋白。反过来，这将有助于阐明SifA在酵母细胞内可能参与的功能途径，并最终用于哺乳动物细胞的实验设计。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. SifA is a Salmonella effector protein required for the formation of Salmonella-induced filaments that is translocated across the vacuolar membrane enclosing the pathogen and is required for its maintenance. Preliminary data from Miller lab revealed a distinct subcellular localization when SifA was expressed in yeast. The goal of this collaboration is to identify potential yeast proteins that might be binding to SifA and controlling its localization. In turn, this will help elucidate the functional pathway SifA might be involved in within the yeast cell and eventually, used for designing experiments in mammalian cells.