HUNTINGTIN PROTEIN INTERACTIONS

亨廷顿蛋白相互作用

• 批准号: 7420735
• 负责人: RICHARD RAMSDEN
• 金额: $ 0.5万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420735
• 关键词: genes; glutamine; intracellular transport; microtubules; protein folding; proteins; tubulin; yeasts

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Huntingtin protein is the product of gene IT15, encompassing 67 exons and is approximately 3150 amino acids in length. An expansion of a repeated stretch of glutamines in the protein is linked to the fatal, autosomal dominant neurodegenerative disorder, Huntington¿¿s disease. Cytoplasmic huntingtin colocalizes with microtubules and interacts with ss-tubulin and evidence may suggests that huntingtin is involved in intracellular transport and may interact with many protein partners. Here, both wild type and aberrant natively folded huntingtin will be used in a systematic screen of yeast ORFs. These data should help elucidate the nature of the natively folded protein.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。亨廷顿蛋白是IT15基因的产物，包含67个外显子，长度约为3150个氨基酸。蛋白质中重复延伸的谷氨酰胺与致命的常染色体显性神经退行性疾病亨廷顿病有关。 细胞质亨廷顿蛋白与微管共定位并与 SS-微管蛋白相互作用，有证据可能表明亨廷顿蛋白参与细胞内运输，并可能与许多蛋白质伙伴相互作用。 在这里，野生型和异常的天然折叠亨廷顿蛋白都将用于酵母 ORF 的系统筛选。这些数据应该有助于阐明天然折叠蛋白质的性质。