MOLECULAR DISSECTION OF THE C ELEGANS KINETOCHORE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们目前正在约翰·耶茨的实验室与伊恩·麦克劳德合作，对线虫和人类动植物进行生化分析。使用各种不同的纯化策略，我们正试图分离所有已知的线虫和人类动粒蛋白，并在此过程中鉴定新的相互作用蛋白。虽然线虫并不是以出色的生化系统而闻名，但结合纯化策略，我们已经能够纯化一些动粒蛋白。我们计划继续这项分析，并最终希望进一步确定这些动粒蛋白中的磷酸化位点。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are currently working with Ian MacLeod in John Yates's lab to conduct a biochemical analysis of the C. elegans and human kinetochores. Using a variety of different purification strategies, we are attempting to isolate all of the known C. elegans and human kinetochore proteins and in the process identify novel interacting proteins. While C. elegans is not renowned for being an excellent biochemical system, the combination of purification strategies has allowed us to purify a number of kinetochore proteins. We plan to continue this analysis and ultimately hope to additionally identify phosphorylation sites in these kinetochore proteins.