IDENTIFICATION OF TOR INTERACTING PROTEINS

Tor 相互作用蛋白的鉴定

• 批准号: 7420664
• 负责人: MARIA E CARDENAS-CORONA
• 金额: $ 0.29万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420664
• 关键词: Saccharomyces cerevisiae; T lymphocyte; evolution; gene expression; human; immune system; immunosuppression; immunosuppressive; isomerase; microorganism growth; play; protein kinase; proteins; ribosomes; role; toxin; yeasts

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are studying the Tor signaling cascade, which senses nutrients and regulates gene expression, translation, and ribosome biogenesis and has been conserved over a billion years of evolution from yeast to humans. The Tor proteins are novel protein kinases whose functions are inhibited by the immunosuppressive antifungal drug rapamycin in complex with the prolyl isomerase FKBP12. We focus on the antiproliferative drug rapamycin, which suppresses the immune system by blocking signaling events required for activation of T-lymphocytes. Rapamycin is used to treat and prevent graft rejection in organ transplant recipients. Rapamycin is a product of a soil bacterium and likely plays a role in nature distinct from immunosuppression, possibly as a toxin to inhibit growth of competing microorganisms. Based on this hypothesis, we have analyzed in detail the mechanisms of rapamycin action in the yeast Saccharomyces cerevisiae. We would like to continue our analysis of the TOR proteins in collaboration with the Yeast Resource Center.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们正在研究Tor信号级联，它可以感知营养物质并调节基因表达，翻译和核糖体生物合成，并且在从酵母到人类的十亿年进化中一直保持不变。Tor蛋白是新型蛋白激酶，其功能被免疫抑制性抗真菌药物雷帕霉素与脯氨酰异构酶FKBP 12复合物抑制。我们专注于抗增殖药物雷帕霉素，它通过阻断T淋巴细胞活化所需的信号事件来抑制免疫系统。 雷帕霉素用于治疗和预防器官移植受者的移植排斥反应。雷帕霉素是一种土壤细菌的产物，在自然界中可能起着与免疫抑制不同的作用，可能作为一种毒素抑制竞争微生物的生长。基于这一假设，我们详细分析了雷帕霉素在酿酒酵母中的作用机制。我们希望继续与酵母资源中心合作分析TOR蛋白。