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The Scatterlings of Virulence: towards a complete type-III secretion effector repertoire in Escherichia coli

毒力的分散：大肠杆菌中完整的 III 型分泌效应器库

基本信息

批准号：
BB/D010195/1
负责人：
Mark Pallen
金额：
$ 41.72万
依托单位：
University of Birmingham
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2006
资助国家：
英国
起止时间：
2006 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FD010195%2F1
关键词：
Scatterlings Virulence towards complete type

项目摘要

Some strains of the bacterium Escherichia coli (or E. coli) can inject proteins into human cells, using a specialized molecular syringe known as a 'type-III secretion system'. Once inside human cells, the bacterial proteins then produce effects that help the bacterium colonize the gut and/or cause disease. Until recently it was thought that only a handful of these so-called 'effector proteins' passed through the molecular syringe. However, we have uncovered evidence from analysis of genome sequences to suggest that as many as a hundred different proteins are injected through the type-III secretion system of one particularly virulent strain, E. coli O157. In other words, instead of a using a sextet of proteins, E. coli is employed a symphony orchestra of proteins to seduce human cells into doing what it wants. These new effector proteins are encoded by genes scattered throughout the E. coli chromosome, hence our evocative epithet, 'the scatterlings of virulence'. In this proposal, we will draw on the combined expertise of a bacteriologist and a cell biologist to confirm that the new candidate effector proteins are indeed targeted into human cells, to determine where they go within these cells and what effects they produce once inside human cells. In so doing we will shed light on how bacteria cause disease and on the normal functioning of the systems they disturb.

一些大肠杆菌菌株（或E.大肠杆菌）可以将蛋白质注入人体细胞，使用一种称为“III型分泌系统”的专门分子注射器。一旦进入人体细胞，细菌蛋白就会产生帮助细菌在肠道定植和/或引起疾病的作用。直到最近，人们还认为只有少数这些所谓的“效应蛋白”通过了分子注射器。然而，我们从基因组序列分析中发现的证据表明，有多达一百种不同的蛋白质通过一种特别强毒株E. coli O157。换句话说，E.大肠杆菌是一个由蛋白质组成的交响乐团，用来引诱人类细胞做它想做的事情。这些新的效应蛋白由散布在大肠杆菌中的基因编码。大肠杆菌染色体，因此我们有一个令人回味的绰号，“散布的毒力”。在这项提案中，我们将利用细菌学家和细胞生物学家的综合专业知识来确认新的候选效应蛋白确实靶向人类细胞，以确定它们在这些细胞中的位置以及它们在人类细胞内产生的影响。通过这样做，我们将阐明细菌如何引起疾病以及它们干扰的系统的正常功能。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

An extensive repertoire of type III secretion effectors in Escherichia coli O157 and the role of lambdoid phages in their dissemination

DOI：
10.1073/pnas.0604891103
发表时间：
2006-10-03
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Tobe, Toru;Beatson, Scott A.;Pallen, Mark J.
通讯作者：
Pallen, Mark J.

Enterohemorrhagic Escherichia coli exploits EspA filaments for attachment to salad leaves.

肠出血性大肠杆菌利用 EspA 细丝附着在沙拉叶上。