Surface-Enhanced Raman Glucose Sensor
表面增强拉曼血糖传感器
基本信息
- 批准号:7686651
- 负责人:
- 金额:$ 26.8万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-09-15 至 2010-07-31
- 项目状态:已结题
- 来源:
- 关键词:AcuteAddressAdsorptionAlgorithmsAmericanAnesthesia proceduresAnimalsArchitectureAreaAscorbic AcidBehaviorBiologicalBiological Neural NetworksBiological PreservationBiosensorBlood VesselsBoronic AcidsCalibrationChemistryChronic DiseaseClassCollectionDataData AnalysesData SetDepositionDetectionDevicesDiabetes MellitusDiamondDiseaseDoseEyeFiber OpticsFilmGlucoseGlycerolGlycolGoalsHypoglycemiaImmunohistochemistryImplantInfiltrationInflammationInfusion proceduresInsulinIntercellular FluidInvasiveKidneyLasersLeukocytesLiquid ChromatographyLiquid substanceMass Spectrum AnalysisMeasurementMeasuresMetabolicMethodsModalityModelingModificationMonitorNanosphereNerveObject AttachmentOperative Surgical ProceduresOpticsPartition CoefficientPatientsPerformancePersonsPolyethylene GlycolsProcessProteinsPublic HealthRaman Spectrum AnalysisRattusReaction TimeRecoveryResearchResistanceSamplingSignal TransductionSilicon DioxideSkinSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSpectrum AnalysisSprague-Dawley RatsStandards of Weights and MeasuresSterilitySurfaceSurface Plasmon ResonanceSynthesis ChemistryTechniquesTestingThickTimeTissuesTreatment ProtocolsUreaValidationatomic layer depositionawakebasebiomaterial compatibilityblood glucose regulationboronic acidcarboxylatedaydesignglucose monitorglucose sensorimplantationimprovedin vivoliquid chromatography mass spectrometrymonolayernanoparticlenanostructurednovel strategiespreventresponsesensorsmall moleculesubcutaneoustransmission process
项目摘要
Approximately 20 million Americans and ten times that many persons worldwide have diabetes mellitus.
This disease is a chronic disorder that requires careful regulation of glucose levels within tight limits in order to
prevent severe secondary complications involving the patient's eyes, kidneys, nerves, and blood vessels.
Recent research from our group has demonstrated that surface-enhanced Raman spectroscopy (SERS) is a
new approach to this important public health problem that offers significant promise as a means to measure
real-time, in vivo glucose levels. Many of the potential problems envisioned at the outset of this project such
as: (1) glucose had never been measured by SERS; (2) temporal stability; (3) reversibility; (4) real-time
response; (5) resistance to protein interference; and (6) complications from interfering small molecule analytes
have been demonstrated to be non-existent or have been overcome.
The research proposed herein is focused on the remaining fundamental scientific and technical challenges
associated with developing in vivo SERS as robust, portable biosensor platform for glucose in biological fluids.
Four specific aims to designed to achieve this goal are: (1) synthesize and optimize new partition layers that
reduce spectral overlap with target analytes, increase sensitivity, and increase in vivo operating lifetime; (2)
improve the chemometric data analysis and calibration methods used to extract the glucose level from the raw
signal ; (3) develop both fiber optic and free-space excitation/collection approaches to transdermal SERS; and
(4) use a rat model to quantify glucose levels in unrestrained, unanaesthetized animals for periods up to 5
days. New partition layers will be created through synthetic chemistry to minimize spectral overlap. The
operating lifetime of the partition layer will be dramatically increased using a novel approach based on atomic
layer deposition to eliminate the weak Ag-S or Au-S bond. Calibration will be improved by measuring the
glucose partition coefficient using liquid chromatography and by using a robust internal Raman standard
(diamond). Transdermal SERS studies will be carried out to demonstrate the viability of free-space laser
excitation and collection. Preliminary results are presented indicating that this is possible. In vivo SERS will be
cross-validated using an implanted electrochemical sensor (MiniMed) and ex vivo monitoring of glucose by
liquid chromatography/mass spectrometry.
大约有2000万美国人患有糖尿病,全世界有10倍于此的人数。
这种疾病是一种慢性疾病,需要在严格的范围内仔细调节葡萄糖水平,
预防涉及患者眼睛、肾脏、神经和血管的严重继发性并发症。
我们小组最近的研究表明,表面增强拉曼光谱(Sers)是一种
这一重要的公共卫生问题的新方法,提供了重大的承诺,作为衡量
实时体内葡萄糖水平。在这个项目开始时设想的许多潜在问题,
因为:(1)葡萄糖从未被Sers测量过;(2)时间稳定性;(3)可逆性;(4)实时性
反应;(5)对蛋白质干扰的抗性;以及(6)干扰小分子分析物的并发症
已被证明是不存在的或已被克服。
本文提出的研究重点是剩余的基本科学和技术挑战
与开发体内Sers作为生物流体中葡萄糖的稳健便携式生物传感器平台相关。
为实现这一目标而设计的四个具体目标是:(1)合成和优化新的分区层,
减少与目标分析物的光谱重叠,增加灵敏度,并增加体内工作寿命;(2)
改进化学计量数据分析和校准方法,用于从原始数据中提取葡萄糖水平
信号;(3)开发光纤和自由空间激发/收集方法来进行经皮Sers;以及
(4)使用大鼠模型来量化不受限制的、未麻醉的动物中的葡萄糖水平,
天将通过合成化学方法创建新的分区层,以最大限度地减少光谱重叠。的
使用基于原子的新方法,
层沉积以消除弱的Ag-S或Au-S键。校准将通过测量
采用液相色谱法和耐用内标拉曼法测定葡萄糖分配系数
(菱形)。透皮表面Sers的研究将被用来证明自由空间激光的可行性
激发和收集。初步结果表明,这是可能的。在体内Sers将是
使用植入式电化学传感器(MiniMed)交叉验证,并通过
液相色谱/质谱法。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(1)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
RICHARD Palmer VAN DUYNE其他文献
RICHARD Palmer VAN DUYNE的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('RICHARD Palmer VAN DUYNE', 18)}}的其他基金
相似海外基金
Rational design of rapidly translatable, highly antigenic and novel recombinant immunogens to address deficiencies of current snakebite treatments
合理设计可快速翻译、高抗原性和新型重组免疫原,以解决当前蛇咬伤治疗的缺陷
- 批准号:
MR/S03398X/2 - 财政年份:2024
- 资助金额:
$ 26.8万 - 项目类别:
Fellowship
CAREER: FEAST (Food Ecosystems And circularity for Sustainable Transformation) framework to address Hidden Hunger
职业:FEAST(食品生态系统和可持续转型循环)框架解决隐性饥饿
- 批准号:
2338423 - 财政年份:2024
- 资助金额:
$ 26.8万 - 项目类别:
Continuing Grant
Re-thinking drug nanocrystals as highly loaded vectors to address key unmet therapeutic challenges
重新思考药物纳米晶体作为高负载载体以解决关键的未满足的治疗挑战
- 批准号:
EP/Y001486/1 - 财政年份:2024
- 资助金额:
$ 26.8万 - 项目类别:
Research Grant
Metrology to address ion suppression in multimodal mass spectrometry imaging with application in oncology
计量学解决多模态质谱成像中的离子抑制问题及其在肿瘤学中的应用
- 批准号:
MR/X03657X/1 - 财政年份:2024
- 资助金额:
$ 26.8万 - 项目类别:
Fellowship
CRII: SHF: A Novel Address Translation Architecture for Virtualized Clouds
CRII:SHF:一种用于虚拟化云的新型地址转换架构
- 批准号:
2348066 - 财政年份:2024
- 资助金额:
$ 26.8万 - 项目类别:
Standard Grant
The Abundance Project: Enhancing Cultural & Green Inclusion in Social Prescribing in Southwest London to Address Ethnic Inequalities in Mental Health
丰富项目:增强文化
- 批准号:
AH/Z505481/1 - 财政年份:2024
- 资助金额:
$ 26.8万 - 项目类别:
Research Grant
ERAMET - Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
ERAMET - 快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10107647 - 财政年份:2024
- 资助金额:
$ 26.8万 - 项目类别:
EU-Funded
BIORETS: Convergence Research Experiences for Teachers in Synthetic and Systems Biology to Address Challenges in Food, Health, Energy, and Environment
BIORETS:合成和系统生物学教师的融合研究经验,以应对食品、健康、能源和环境方面的挑战
- 批准号:
2341402 - 财政年份:2024
- 资助金额:
$ 26.8万 - 项目类别:
Standard Grant
Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10106221 - 财政年份:2024
- 资助金额:
$ 26.8万 - 项目类别:
EU-Funded
Recite: Building Research by Communities to Address Inequities through Expression
背诵:社区开展研究,通过表达解决不平等问题
- 批准号:
AH/Z505341/1 - 财政年份:2024
- 资助金额:
$ 26.8万 - 项目类别:
Research Grant