High Throughput Fractionation and Concentration System for MALDI-MS

描述（由申请人提供）：用于MALDI-MS的高通量分离和浓缩系统这个小型企业创新研究（SBIR）第二期项目旨在建立在第一期可行性研究成功完成的基础上，优化和构建一个原型系统，用于平行制备多达96个血清样本，用于基质辅助激光脱附电离（MALDI）质谱（MS）。该系统被称为MALDIplex(tm) M5样品准备工作站，由台式仪器、图形用户界面（GUI）和一次性96孔样品盒组成。MALDIplex套管是专门设计的，通过一系列堆叠的薄层支撑，可以实现每口井的平行垂直分离。该系统允许集成的样品纯化，分馏，浓缩和脱盐-所有在一个单一的，易于使用的墨盒。使用该系统，样品可以在不到45分钟内同时制备。没有其他系统能提供这个系统所能达到的潜在吞吐量和数据质量。第二阶段的工作将集中在进一步发展和优化定量和分离方案，以及改进系统设计（自动化）和从药筒表面鉴定蛋白质的方法。这项创新技术有可能在提高对癌症等复杂疾病的理解、诊断、预后和治疗方面发挥关键作用。如果成功，第二阶段将产生一个优化的样品制备系统，为第三阶段的全面生产和商业化做好准备。7. 利用质谱法寻找新的药物靶点和疾病标志物（生物标志物）在提高对疾病的认识、诊断、预后和治疗方面具有巨大的潜力。许多复杂的、危及生命的疾病，如癌症，需要早期诊断以最大限度地有效治疗。如果成功，这个II期研究项目将使大规模的、临床相关的生物标志物发现使用质谱，这有可能揭示新的、高灵敏度的生物标志物，用于早期疾病诊断和预测治疗反应。

DESCRIPTION (provided by applicant): High Throughput Fractionation and Concentration System for MALDI-MS This Small Business Innovative Research (SBIR) Phase II project aims to build upon the successful work completed in the Phase I feasibility study to optimize and construct a prototype system for the preparation of up to 96 serum samples in parallel for matrix assisted laser desorption ionization (MALDI) mass spectrometry (MS). This system, called the MALDIplex(tm) M5 Sample Prep Station, consists of a benchtop instrument, a graphical user interface (GUI), and single-use 96-well cartridges. The MALDIplex cartridges are specially designed to permit parallel vertical separation from each well through a series of stacked thin layer supports. The system permits integrated sample purification, fractionation, concentration, and desalting - all in a single, easy to use cartridge. Using this system, samples may be prepared simulaneously in less than 45 minutes. No other system offers the potential throughput and data quality achievable with this system. Phase II efforts will focus on the further development and optimization of quantitation and fractionation protocols, as well as improved system designs (for automation) and methods for protein identification from the cartridge surface. This innovative technology has the potential to play a key role in improving the understanding, diagnosis, prognosis, and treatment of complex disease such as cancer. If successful, the Phase II will result in an optimized sample preparation system ready for full scale manufacturing and commercialization in Phase III. 7. Project Narrative High Throughput Fractionation and Concentration System for MALDI-MS The search of novel drug targets and disease markers (biomarkers) using mass spectrometry holds tremendous potential for improving the understanding, diagnosis, prognosis, and treatment of disease. Many complex, life threatening diseases, such as cancer, require early diagnosis to maximize effective treatment. If successful, this Phase II research project will enable large scale, clinically-relevant biomarker discovery using mass spectrometry, which has the potential of revealing new, highly sensitive biomarkers for early disease diagnosis and to predict treatment response.