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Neuropathology and Pathogenesis of Huntington's Disease

亨廷顿病的神经病理学和发病机制

基本信息

批准号：
7657929
负责人：
ANTON J. REINER
金额：
$ 32.19万
依托单位：
UNIVERSITY OF TENNESSEE HEALTH SCI CTR
依托单位国家：
美国
项目类别：
财政年份：
1990
资助国家：
美国
起止时间：
1990-07-15 至 2010-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7657929
关键词：
Address Affect Animals Axonal Transport BAD gene Bad protein Basal Ganglia Brain Diseases Brain-Derived Neurotrophic Factor Cell physiology Cerebral cortex Cessation of life Cholinergic Agents Cognitive Corpus striatum structure Dependence Disease Functional disorder Human Huntington Disease Impaired cognition Implant In Vitro Induced Mutation Inherited Injury Interneurons Knock-out Label MEKs Mediating Motor Mus Mutant Strains Mice Mutate Mutation Neurodegenerative Disorders Neurons Neurotrophic Tyrosine Kinase Receptor Type 2 Pathogenesis Pathology Pathway interactions Pattern Phosphorylation Play Process Production Pyramidal Tracts Replacement Therapy Role Signal Pathway Signal Transduction Site Somatostatin Staging Stem cells Transgenic Mice Transgenic Organisms Western Blotting cellular engineering cholinergic deprivation disease-causing mutation human Huntingtin protein injured motor control mutant mutant mouse model neuron loss neuropathology pro-apoptotic protein pro-caspase-9 protein expression

项目摘要

Huntington¿s disease (HD) is a hereditary neurodegenerative disorder that results in a progressive decline in cognitive ability due to an early occurring dysfunction in cerebral cortex and a progressive decline in motor control due to the steady loss of striatal projection neurons from the basal ganglia. While the mutated huntingtin protein (Htt) that underlies HD pathogenesis appears to interfere with a wide array of cellular functions, the means by which mutant Htt brings about the cortical and striatal pathology of HD remains uncertain. Several lines of evidence suggest that the Htt mutation causes a reduction in BDNF (brain-derived neurotrophic factor) production and transport by corticostriatal neurons, resulting in deprivation of striatal neurons of this vital trophic factor, and that this may be the major means by which HD destroys striatal projection neurons. It is unknown, however, if the striatal pathology caused by BDNF deprivation is truly HD-like, and it is unknown if the HD mutation initiates a striatal injury process that is mediated by a deficit in pro-survival BDNF signaling. The present proposal seeks to address these two issues to more firmly establish if BDNF deprivation plays a significant role in the striatal injury process in HD. If shown to be implicated, BDNF replacement therapy would then be a viable approach for combating striatal injury in HD, for example by means of striatal implant of stem cells engineered to produce BDNF. Moreover, if the cerebral cortex is identified by our studies as the primary site at which the mutation acts to bring about indirect striatal injury, our findings would guide therapies seeking to reduce mutant protein expression to target cortex. Four lines of study will be carried out, each to address a key question related to the hypothesis that striatal deprivation of cortically produced BDNF underlies striatal injury in HD. Aim 1. Are the localization of BDNF in corticostriatal neurons and its receptor trkB in striatal projection neurons in normal animals consistent with the differential vulnerability among striatal projection neurons in HD? Aim 2. Is the vulnerability of striatal projection neurons to BDNF deprivation consistent with their differential vulnerability in HD? Aim 3. What intracellular signaling pathways mediate the deleterious effects of BDNF deprivation on striatal projection neurons? Aim 4. Does the HD mutation cause injury to striatal projection neurons via the intracellular signaling pathways used by BDNF deprivation? The studies will employ mutant mouse models of HD and cortex-specific knockout of BDNF expression, and in vitro approaches.

亨廷顿氏病（HD）是一种遗传性神经退行性疾病，导致进行性神经退化