Core--Environmental Molecular Analysis
核心--环境分子分析
基本信息
- 批准号:7064120
- 负责人:
- 金额:$ 23.1万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-04-01 至 2011-03-31
- 项目状态:已结题
- 来源:
- 关键词:aromatic hydrocarbon receptorbenzofuransbioinformaticsbiomedical facilitybiotechnologycarbopolycyclic compounddioxinsenvironmental contaminationgenetic libraryhalobiphenyl /halotriphenyl compoundhigh throughput technologymathematical modelmicroarray technologymodel design /developmentmolecular biologynucleic acid sequencepolymerase chain reaction
项目摘要
Molecular technologies have become key to a more in-depth understanding of the complex processes and
interactions of microbial communities, and are especially important to a mechanistic understanding of
bioremediation processes. New tools are constantly being developed to aid this objective but their
application and optimization to microbial research under environmentally relevant conditions is not easy.
Our overall goal is to use and enhance these technologies for understanding how microbes react to
environmental changes in situ rather than simply as laboratory pure cultures. This can best be done by a
support core with interacting components. We will develop new tools and
discover new genes involved in degradation of polyaromatic compounds. This process will require the
support of a variety of enabling technologies, the most central of which are provided and optimized in this
support core. The specific aims of this core proposal are to provide needed support in three related areas:
(1) microarray development and enhancement, (2) automated bioinformatic analyses of PCR product
sequences and biodegradative gene clusters, and (3) high throughput screening and sequencing of
environmental clones. The project brings together the strengths of a multidisciplinary team of researchers,
each one acting in their own areas of expertise. The Center for Microbial Ecology (CME) and the
Department of Civil and Environmental Engineering at Michigan State University has developed a microarray
platform that will support our proposed genomic analyses. CME's Microbial Informatics Group manages the
Ribosomal Database Project and has developed a bioinformatic platform for sequence analyses and other
data analysis tools. The Biotechnology Center for Agriculture and the Environment at Rutgers University has
developed a high throughput screening facility with an emphasis on screening cultures and clones. These
three components will form an interacting triad supporting the environmental research projects, and will
exchange at a general level strategies and concepts with the Biomedical Informatics core and toxicology
projects that use genetic and microarray array technologies.
分子技术已成为更深入了解复杂过程的关键,
微生物群落的相互作用,特别是重要的机械理解,
生物修复过程。不断开发新的工具来帮助实现这一目标,
在环境相关条件下对微生物研究的应用和优化并不容易。
我们的总体目标是使用和增强这些技术,以了解微生物如何对
环境的变化,而不是简单地作为实验室纯培养。这最好由一个
支撑具有相互作用组件的核心。我们将开发新的工具,
发现参与多环芳烃化合物降解的新基因。这一过程将需要
支持各种使能技术,其中最核心的技术是在这方面提供和优化的。
支持核心。这一核心提案的具体目标是在三个相关领域提供所需的支助:
(1)微阵列的开发和增强,(2)PCR产物的自动生物信息学分析
序列和生物降解基因簇,和(3)高通量筛选和测序
环境克隆该项目汇集了一个多学科研究团队的力量,
每个人都在自己的专业领域工作。微生物生态学中心(CME)和
密歇根州立大学土木与环境工程系开发了一种微阵列
该平台将支持我们提出的基因组分析。CME的微生物信息学小组管理着
核糖体数据库项目,并开发了一个生物信息学平台,用于序列分析和其他
数据分析工具。罗格斯大学农业和环境生物技术中心
开发了高通量筛选设施,重点是筛选培养物和克隆。这些
三个组成部分将形成一个相互作用的三位一体,支持环境研究项目,并将
在一般水平上与生物医学信息学核心和毒理学交换策略和概念
使用基因和微阵列技术的项目。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
GERBEN J ZYLSTRA其他文献
GERBEN J ZYLSTRA的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('GERBEN J ZYLSTRA', 18)}}的其他基金
Molecular Insight into Toxicant Degradation by Microbial Communities
微生物群落有毒物质降解的分子洞察
- 批准号:
7596967 - 财政年份:2007
- 资助金额:
$ 23.1万 - 项目类别:
Molecular Insight into Toxicant Degradation by Microbial Communities
微生物群落有毒物质降解的分子洞察
- 批准号:
7459914 - 财政年份:2007
- 资助金额:
$ 23.1万 - 项目类别:
Molecular Insight into Toxicant Degradation by Microbial Communities
微生物群落有毒物质降解的分子洞察
- 批准号:
7290870 - 财政年份:2007
- 资助金额:
$ 23.1万 - 项目类别:
ELECTRON TRANSFER BETWEEN TOLUENE DIOXYGENASE COMPONENTS
甲苯双加氧酶组分之间的电子转移
- 批准号:
3044120 - 财政年份:1989
- 资助金额:
$ 23.1万 - 项目类别:
ELECTRON TRANSFER BETWEEN TOLUENE DIOXYGENASE COMPONENTS
甲苯双加氧酶组分之间的电子转移
- 批准号:
3044121 - 财政年份:1988
- 资助金额:
$ 23.1万 - 项目类别:
Project 5: Molecular Insight into Dioxin Degradation by Microbes and Microbial
项目 5:微生物和微生物降解二恶英的分子洞察
- 批准号:
8564240 - 财政年份:
- 资助金额:
$ 23.1万 - 项目类别:
Project 5: Molecular Insight into Dioxin Degradation by Microbes and Microbial
项目 5:微生物和微生物降解二恶英的分子洞察
- 批准号:
8695355 - 财政年份:
- 资助金额:
$ 23.1万 - 项目类别:
相似海外基金
A Concise, Enantioselective Approach to the Synthesis of (-)-Rhodomollanol-A
(-)-红多酚-A 的简洁、对映选择性合成方法
- 批准号:
10436840 - 财政年份:2021
- 资助金额:
$ 23.1万 - 项目类别:
Optimization of antimalarials targeting multiple life stages of the parasite
针对寄生虫多个生命阶段的抗疟药物的优化
- 批准号:
10298005 - 财政年份:2021
- 资助金额:
$ 23.1万 - 项目类别:
Optimization of antimalarials targeting multiple life stages of the parasite
针对寄生虫多个生命阶段的抗疟药物的优化
- 批准号:
10813425 - 财政年份:2021
- 资助金额:
$ 23.1万 - 项目类别:
Optimization of antimalarials targeting multiple life stages of the parasite
针对寄生虫多个生命阶段的抗疟药物的优化
- 批准号:
10689033 - 财政年份:2021
- 资助金额:
$ 23.1万 - 项目类别:
Chemistry and Pharmacology of Iboga Alkaloids
Iboga 生物碱的化学和药理学
- 批准号:
10179354 - 财政年份:2020
- 资助金额:
$ 23.1万 - 项目类别:
Chemistry and Pharmacology of Iboga Alkaloids
Iboga 生物碱的化学和药理学
- 批准号:
10594417 - 财政年份:2020
- 资助金额:
$ 23.1万 - 项目类别:
Chemistry and Pharmacology of Iboga Alkaloids
Iboga 生物碱的化学和药理学
- 批准号:
10370433 - 财政年份:2020
- 资助金额:
$ 23.1万 - 项目类别:
The Next Generation of Hallucinogens: A New Class of Synthetic Psychoactive Drugs
下一代致幻剂:一类新型合成精神活性药物
- 批准号:
9238460 - 财政年份:2017
- 资助金额:
$ 23.1万 - 项目类别:
Synthesis and Evaluation of DIM-like Analogues Targeting PI3K/Akt Pathway
靶向 PI3K/Akt 通路的 DIM 类似物的合成和评价
- 批准号:
8552026 - 财政年份:2013
- 资助金额:
$ 23.1万 - 项目类别: