Core--Environmental Molecular Analysis

核心--环境分子分析

• 批准号: 7466408
• 负责人: GERBEN J ZYLSTRA
• 金额: $ 23.19万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7466408
• 关键词: Agriculture; Area; Bioinformatics; Bioremediations; Biotechnology; Burkholderia; Complex; Condition; Data; Data Analyses; Databases; Depth; Development; Ecology; Environment; Environmental Engineering technology; Equipment; Gene Cluster; Gene Family; Genes; Genetic; Genomics; Goals; In Situ; Informatics; Label; Laboratories; Libraries; Manuals; Michigan; Microbe; Modeling; Molecular; Molecular Analysis; Open Reading Frames; Polymerase Chain Reaction; Process; Research; Research Personnel; Research Project Grants; Ribosomes; Running; Sampling; Screening procedure; Sequence Analysis; Services; Staging; Statistical Models; Technology; Toxicology; Transcript; Triad Acrylic Resin; Universities; Work; biomedical informatics; concept; cost; design; environmental change; experience; high throughput screening; microbial; microbial community; microbial genome; multidisciplinary; new technology; repository; research study; statistics; tool; tool development

Molecular technologies have become key to a more in-depth understanding of the complex processes and interactions of microbial communities, and are especially important to a mechanistic understanding of bioremediation processes. New tools are constantly being developed to aid this objective but their application and optimization to microbial research under environmentally relevant conditions is not easy. Our overall goal is to use and enhance these technologies for understanding how microbes react to environmental changes in situ rather than simply as laboratory pure cultures. This can best be done by a support core with interacting components. We will develop new tools and discover new genes involved in degradation of polyaromatic compounds. This process will require the support of a variety of enabling technologies, the most central of which are provided and optimized in this support core. The specific aims of this core proposal are to provide needed support in three related areas: (1) microarray development and enhancement, (2) automated bioinformatic analyses of PCR product sequences and biodegradative gene clusters, and (3) high throughput screening and sequencing of environmental clones. The project brings together the strengths of a multidisciplinary team of researchers, each one acting in their own areas of expertise. The Center for Microbial Ecology (CME) and the Department of Civil and Environmental Engineering at Michigan State University has developed a microarray platform that will support our proposed genomic analyses. CME's Microbial Informatics Group manages the Ribosomal Database Project and has developed a bioinformatic platform for sequence analyses and other data analysis tools. The Biotechnology Center for Agriculture and the Environment at Rutgers University has developed a high throughput screening facility with an emphasis on screening cultures and clones. These three components will form an interacting triad supporting the environmental research projects, and will exchange at a general level strategies and concepts with the Biomedical Informatics core and toxicology projects that use genetic and microarray array technologies.

分子技术已成为对复杂过程的更深入理解和 微生物群落的相互作用，对于机械理解尤其重要 生物修复过程。不断开发新工具以帮助这一目标，但是他们 在环境相关条件下对微生物研究的应用和优化并不容易。 我们的总体目标是利用和增强这些技术来了解微生物如何反应 环境变化原位，而不是简单地作为实验室纯文化。最好通过 用相互作用组件支持核心。我们将开发新工具， 发现涉及多野型化合物降解的新基因。这个过程需要 支持各种支持技术，其中最中心是在此提供和优化的 支持核心。该核心建议的具体目的是在三个相关领域提供所需的支持： （1）微阵列的开发和增强，（2）PCR产品的自动生物信息学分析 序列和生物降解基因簇，以及（3）高吞吐量筛选和测序 环境克隆。该项目汇集了一个多学科研究人员的优势， 每个人都在自己的专业领域中行事。微生物生态学中心（CME）和 密歇根州立大学的民用与环境工程系已开发了一个微阵列 将支持我们提出的基因组分析的平台。 CME的微生物信息学组管理 核糖体数据库项目，并开发了一个生物信息学平台，用于序列分析和其他 数据分析工具。罗格斯大学的农业与环境生物技术中心有 开发了高通量筛查设施，重点是筛选培养物和克隆。这些 三个组件将形成一个互动的三合会，以支持环境研究项目，并将 与生物医学信息学核心和毒理学的一般策略和概念进行交流 使用遗传和微阵列阵列技术的项目。