HIGH RESOLUTION SPECTROSCOPIC STUDIES IN AMRIS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。在500、600和750 MHz仪器上使用更传统的光谱学进行了各种光谱学研究，但借助AMRIS相关教职员工的专业知识，上面仅列出了相关的AMRIS相关MR教职员工。这些研究包括：酿酒酵母天冬氨酸蛋白酶抑制剂IA3的研究(证明在溶液中本质上是非结构的)；果蝇动力蛋白轻链LC8在pH诱导下单体的溶液结构的测定；抗生素Mutacin1140的结构和动力学的测定；禽类Thy1在Lec1哺乳动物和Tn5昆虫细胞中的表达、纯化和鉴定；小鼠黑皮质素受体上黑皮质素四肽Ac-His-DPhe-Arg-Trp-NH2的构效关系的测定；以及黑素皮质素受体上脂族、环状和N-末端“封顶”的His-D-Phe-Arg-Trp-NH2四肽的鉴定。预计这些研究的各个方面将利用在本资源中开发的微线圈和HTS技术，当它们成为成熟的、蓬勃发展的合作项目时。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A variety of spectroscopy studies are performed on the 500, 600 and 750 MHz instruments using more conventional spectroscopy, but drawing upon the expertise of the AMRIS associated faculty and staff ¿ the relevant AMRIS associated MR faculty and staff only are listed above. These include; studies of IA3, an aspartic proteinase inhibitor from Saccharomyces cerevisiae (shown to be intrinsically unstructured in solution; determination of the solution structure of the pH-induced monomer of dynein light chain LC8 from Drosophila; determination of the structure and dynamics of the Lantibiotic Mutacin 1140; expression, purification, and characterization of avian Thy1 from Lec1 mammalian and Tn5 insect cells; determination of the structure-activity relationships of the melanocortin tetrapeptide Ac-His-DPhe-Arg-Trp-NH2 at the mouse melanocortin receptors; and characterization of aliphatic, cyclic, and aromatic N-terminally "capped" His-D-Phe-Arg-Trp-NH2 tetrapeptides at the melanocortin receptors. It is anticipated that aspects of these studies will make use of the microcoil and HTS technologies developed in this Resource when they become mature, beoming collaborative projects.