HIGH RESOLUTION SPECTROSCOPIC STUDIES IN AMRIS

AMRIS 中的高分辨率光谱研究

• 批准号: 7369587
• 负责人: ARTHUR S EDISON
• 金额: $ 0.58万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7369587
• 关键词: RESOLUTION; SPECTROSCOPIC; STUDIES; AMRIS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A variety of spectroscopy studies are performed on the 500, 600 and 750 MHz instruments using more conventional spectroscopy, but drawing upon the expertise of the AMRIS associated faculty and staff ¿ the relevant AMRIS associated MR faculty and staff only are listed above. These include; studies of IA3, an aspartic proteinase inhibitor from Saccharomyces cerevisiae (shown to be intrinsically unstructured in solution; determination of the solution structure of the pH-induced monomer of dynein light chain LC8 from Drosophila; determination of the structure and dynamics of the Lantibiotic Mutacin 1140; expression, purification, and characterization of avian Thy1 from Lec1 mammalian and Tn5 insect cells; determination of the structure-activity relationships of the melanocortin tetrapeptide Ac-His-DPhe-Arg-Trp-NH2 at the mouse melanocortin receptors; and characterization of aliphatic, cyclic, and aromatic N-terminally "capped" His-D-Phe-Arg-Trp-NH2 tetrapeptides at the melanocortin receptors. It is anticipated that aspects of these studies will make use of the microcoil and HTS technologies developed in this Resource when they become mature, beoming collaborative projects.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。使用更传统的光谱学在 500、600 和 750 MHz 仪器上进行各种光谱学研究，但利用 AMRIS 相关教职人员和工作人员的专业知识 - 上面仅列出了相关的 AMRIS 相关 MR 教职人员和工作人员。这些包括； IA3 的研究，一种来自酿酒酵母的天冬氨酸蛋白酶抑制剂（显示在溶液中本质上是非结构化的；测定来自果蝇的 pH 诱导的动力蛋白轻链 LC8 单体的溶液结构；测定 Lantibiotic Mutacin 1140 的结构和动力学；来自 Lec1 哺乳动物和哺乳动物的禽类 Thy1 的表达、纯化和表征 Tn5昆虫细胞；确定黑皮质素四肽 Ac-His-DPhe-Arg-Trp-NH2 在小鼠黑皮质素受体上的构效关系；以及黑皮质素受体上脂肪族、环状和芳香族 N 末端“加帽”His-D-Phe-Arg-Trp-NH2 四肽的表征。预计这些研究的各个方面将 当本资源中开发的微线圈和高温超导技术变得成熟并成为协作项目时，可以利用它们。