The Role of Separase During Cytokinesis in C elegans

分离酶在秀丽隐杆线虫细胞分裂过程中的作用

• 批准号: 7578442
• 负责人: Joshua Nathaniel Bembenek
• 金额: $ 2.52万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2008-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7578442
• 关键词: Affect; Alleles; Anaphase; Animals; Behavior; Biological; Caenorhabditis elegans; Cell Division Process; Cell physiology; Cells; Chromosome Segregation; Chromosomes; Condition; Cytokinesis; Cytoplasmic Structures; Defect; Embryo; Endopeptidases; Ensure; Failure; Fright; Genetic; Genetic Materials; Genetic Nondisjunction; Homologous Gene; Laboratories; Learning; Localized; Mechanics; Meiosis; Mitotic; Molecular; Osmotic Pressure; Pathway interactions; Peptide Hydrolases; Range; Role; Saccharomycetales; Signal Pathway; Sister Chromatid; Structure; Techniques; Temperature; Time; cohesion; mutant; separase; transmission process

The process of cell division requires the correct sequence and timing of various cellular processes to ensure faithful transmission of the genetic material and cellular contents. The entry to anaphase occurs upon the cleavage of sister-chromatid cohesion by a protease known as separase. Separase has recently been shown to promote anaphase spindle dynamics and cytokinesis concomitantly with chromosome segregation in budding yeast. Previous studies from our laboratory identified a temperature-sensitive allele of the separase homologue in C. elegans. Embryos from these separase-mutant worms shifted to the restrictive temperature display several meiotic and mitotic defects such as chromosome nondisjunction, osmotic and mechanical sensitivity, and cytokinesis failure. Using various genetic and cell biological techniques, I propose to uncover the mechanisms by which separase contributes to the completion of cytokinesis in the early embryo of C. elegans. Through this analysis, I hope to extend the understanding of the molecular mechanisms utilized for the temporal coordination of chromosome segregation with the onset of anaphase and cytokinesis.

细胞分裂的过程需要各种细胞过程的正确顺序和时间，以确保 遗传物质和细胞内容的忠实传递。进入后期发生在 被称为分离酶的蛋白酶切割姐妹染色单体的凝聚力。分离最近被 显示促进后期纺锤体动力学和伴随染色体分离的胞质分裂 在芽殖酵母中。我们实验室以前的研究确定了一个温度敏感的等位基因， 分离酶同源物在C.优美的这些分离酶突变蠕虫的胚胎转移到限制性的 温度显示出几种减数分裂和有丝分裂缺陷，如染色体不分离、渗透和 机械敏感性和胞质分裂失败。利用各种遗传和细胞生物学技术， 建议揭示分离酶有助于完成胞质分裂的机制， C.早期胚胎优美的通过这一分析，我希望能扩展对分子的理解， 染色体分离与后期开始的时间协调机制 和胞质分裂。