Evolution of bric-a-brac regulation in Drosphilinae

果蝇亚科小摆设调节的进化

• 批准号: 7333261
• 负责人: Thomas M Williams
• 金额: $ 5.04万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7333261
• 关键词: Abdomen; Animals; Architecture; Binding Sites; Biology; Development; Drosophila genus; Drosophila melanogaster; Elements; Enhancers; Evolution; Fellowship; Female; Functional RNA; Gene Expression Regulation; Genes; Genetic; Green Fluorescent Proteins; Individual; Medical; Modification; Molecular; Mono-S; Morphology; Mutate; Mutation; Names; Nucleic Acid Regulatory Sequences; Pattern; Phenotype; Phylogeny; Pigmentation physiologic function; Pigments; Proteins; Regulation; Regulatory Element; Reporter; Repression; Site; Subgroup; System; Testing; Vertebrates; base; in vivo; insight; interest; male; prevent; sex; sex determination; trait; transcription factor

The field of evolutionary biology has long been interested in the molecular basis of morphological evolution; however a clear understandingremains elusive. Abdominal pigmentation in the subgroup Drosophilinae is a well studiedtrait suitable for studies to identify the genetic changes culminating in the emergence of similar and diversepigmentation patterns. In addition to diverse pigmentation patternsit is apparent, based on phylogeny, that similar patternsof pigmentation haveappeared independently. In the melanogaster speciesgroup sexually dimorphic, segmentspecific pigmentation has recently evolved. The terminal abdominal segmentsof males are dark, whereas all segmentsare lightly pigmentedin females. Closely related groups of species are monomorphic, i.e. all segments are lightly pigmented in both sexes. In all species, pigmentation is prevented where the gene products of bric-a-brac (bab), a represserof pigmentation, are expressed. In dimorphic species, a newly evolved circuitry involving homeotic and sex-determination inputs repressesbab expression in the terminal segments of the male, but not the female. This proposal will analyze how Drosophila melanogaster bab gene regulatory elements has evolvedto integrate responsiveness to homeotic and sex-determination inputs and the extent that both similar and diverse abdominal pigmentation patterns haveevolved through the modification of orthologous bab sequences. Insight from the mechanisms of enhancer evolution and function in Drosophilinae will very likely apply to the understandingof cis-regulatoryelements in vertebrates as similar sequence alterationshave significant medical implications inhumans.

长期以来，进化生物学领域一直对形态进化的分子基础感兴趣。然而 明确的了解难以捉摸。亚组果蝇中的腹部色素沉着是一个很好的研究 适合研究以确定遗传变化最终出现相似和多样性化的遗传变化 模式。基于系统发育，显而易见的是色素沉着模式。 色素沉着独立进行。在Melanogaster物种群中，性二态，segments特定于 色素沉着最近进化了。雄性腹部腹部末端是黑暗的，而所有节目 轻微的色素女性。物种紧密相关的组是单态的，即所有片段都是轻轻的色素 在两个性别中。在所有物种中，都可以防止色素沉着，而抑制剂的基因产物（BAB）的基因产物 色素沉着表示。在二态物种中，涉及同性恋和性别的新进化的电路 输入在男性的末端段中抑制了表达，而不是女性。该建议将分析 果蝇Melanogaster BAB基因调节元件如何发展为综合对同源和的响应能力 性别确定的输入以及相似和多样化的腹部色素沉着模式的程度 通过直系同源BAB序列的修饰。从增强子进化和 果蝇中的功能很可能适用于脊椎动物中的顺式调节元素的理解 序列改变具有重要的医学意义。

项目成果

The regulation and evolution of a genetic switch controlling sexually dimorphic traits in Drosophila.

• DOI: 10.1016/j.cell.2008.06.052
• 发表时间: 2008-08-22
• 期刊: CELL
• 影响因子: 64.5
• 作者: Williams, Thomas M.;Selegue, Jane E.;Werner, Thomas;Gompel, Nicolas;Kopp, Artyom;Carroll, Sean B.
• 通讯作者: Carroll, Sean B.