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Exposure to host resistance drives evolution of bacterial virulence in plants; investigating the excision and mobility of genomic island PPHGI-1

暴露于宿主抗性会导致植物中细菌毒力的进化；

基本信息

批准号：
BB/E001998/1
负责人：
Dawn Arnold
金额：
$ 35.27万
依托单位：
University of the West of England
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2007
资助国家：
英国
起止时间：
2007 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FE001998%2F1
关键词：
Exposure host resistance drives evolution

项目摘要

The ability of bacteria to infect animal and plant hosts is determined by proteins known as pathogenicity factors. These factors are encoded by genes, which are frequently found on mobile pieces of DNA known as genomic islands (GI). Transmission of GIs between bacteria is predicted to disseminate pathogenicity determinants amongst bacteria causing the spread of virulence. In plant pathogenic bacteria the acquisition or loss of pathogenicity determinants can result in a change of virulence. We have previously found that the pathogenicity determinant avrPphB resides on a GI designated PPHGI-1, which if deleted from the chromosome of the plant pathogen Pseudomonas syringae pathovar phaseolicola strain 1302A, causes a change in pathogenicity on differential cultivars of bean. Located on the GI are genes predicted to mediate the mobilisation of the GI as well as genes thought to be involved in conjugation of mobile elements from one bacterium to another. This proposal will investigate, using molecular techniques, the function of the genes predicted to mediate movement and conjugation of the PPHGI-1 carrying avrPphB. We hypothesise that these mobility determinants will be involved in movement and transmission of avrPphB. The research will help to elucidate ubiquitous mechanisms involved in the transfer of pathogenicity in plant and animal pathogens, a knowledge of which could impact on medicinal and agricultural treatments against pathogens.

细菌感染动物和植物宿主的能力是由被称为致病因子的蛋白质决定的。这些因子是由基因编码的，这些基因通常存在于被称为基因组岛（GI）的移动的DNA片段上。预计细菌之间的GI传播会在细菌之间传播致病性决定簇，导致毒力传播。在植物病原细菌中，致病性决定因子的获得或丧失可导致毒力的改变。我们先前已经发现，致病性决定子avrPphB位于命名为PPHGI-1的GI上，如果从植物病原体假单胞菌致病变种菜豆生菌株1302 A的染色体上删除，则会导致菜豆不同品种的致病性变化。位于GI上的是预测介导GI移动的基因以及被认为参与移动的元件从一种细菌到另一种细菌的接合的基因。该提案将使用分子技术研究预测介导携带avrPphB的PPHGI-1的运动和结合的基因的功能。我们假设这些流动性决定因素将参与avrPphB的移动和传播。这项研究将有助于阐明植物和动物病原体中致病性转移所涉及的普遍存在的机制，这方面的知识可能会影响针对病原体的医学和农业治疗。