Water splitting in PS II: biophysical studies

PS II 中的水分解：生物物理学研究

• 批准号: 7455115
• 负责人: PETRA FROMME
• 金额: $ 29.63万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7455115
• 关键词: Animals; Atomic Resolution X-Ray Crystallography; Binding; Charge; Chemicals; Complex; Crystallization; Drug Formulations; Electron Spin Resonance Spectroscopy; Electronics; Electrons; Energy Supply; Enzymes; Evolution; Goals; Human; Investigation; Life; Light; Manganese; Membrane; Membrane Proteins; Metals; Modeling; Nuclear; Oxygen; Phase; Photosynthesis; Play; Procedures; Process; Proteins; Protons; Reaction; Resolution; Respiration; Roentgen Rays; Role; Side; Solar Energy; Solubility; Structure; Techniques; Trace Elements; Water; X-Ray Crystallography; base; chemical reaction; cofactor; improved; oxidation; photosystem II; planetary Atmosphere

DESCRIPTION (provided by applicant): Metals play an important role in biocatalysis: more than 20% of all enzymes contain metals in their catalytic centers. One of the most important biometal-catalyzed reactions on earth is the oxidation of water and the release of oxygen catalyzed by the oxygen-evolving complex in Photosystem II. Photosystem II produces all the oxygen in the atmosphere, which humans and animals use for their energy supply by respiration. Photosystem II is a large membrane protein complex embedded in the photosynthetic membrane consisting of 17 proteins to which more than 50 cofactors are bound to perform the complex reactions of light capturing, charge separation and water oxidation. This project has as its main goal the determination of the structure of Photosystem II at atomic resolution and the elucidation of the mechanism of water oxidation by the tetra-nuclear manganese cluster. The physical-chemical parameters of the crystallization process will be determined and the improved crystals will serve as the basis for the determination of the structure of Photosystem II by X-ray crystallography. The electronic structure of the cofactors that are involved in water oxidation will be investigated by spectroscopic investigations using different Electron Paramagnetic Resonance (EPR) techniques on single crystals of Photosystem II. The final goal is to use the structural and functional investigations to unravel the secrets of the mechanism of water oxidation by Photosystem II.

金属在生物催化中起着重要作用：超过20%的酶在其催化中心含有金属。地球上最重要的生物催化反应之一是由光系统II中的放氧复合物催化的水的氧化和氧的释放。光系统II产生大气中所有的氧气，人类和动物通过呼吸提供能量。光系统II是一个大的膜蛋白复合物，它包埋在光合膜中，由17种蛋白质组成，50多种辅因子结合在其上，进行光捕获、电荷分离和水氧化的复杂反应。 该项目的主要目标是以原子分辨率确定光系统II的结构，并阐明四核锰簇氧化水的机制。 将确定结晶过程的物理化学参数，改进的晶体将作为通过X射线晶体学确定光系统II结构的基础。参与水氧化的辅因子的电子结构将通过使用不同的电子顺磁共振（EPR）技术对光系统II的单晶进行光谱研究来研究。 最终的目标是利用结构和功能的调查，以揭开光系统II的水氧化机制的秘密。