COMPARISON OF TRIGLYCERIDES ON PLASMA LEVELS OF BETAHYDROXYBUTERANE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。本初步研究旨在确定中链甘油三酯（MCT）在测量脂肪酸氧化方面是否优于长链甘油三酯（LCT）。脂肪酸是能量的来源，通常这些脂肪在β-氧化过程中在线粒体中转化为乙酰辅酶A。这项评估是由其他研究人员使用脂肪耐量试验进行了10至12小时的间隔。测量β-羟基丁酸酯（BHB）的水平，并计算曲线下面积。该方法在检测健康受试者和家族性混合型高脂血症受试者之间约15%至20%的显著差异中是有用的。此外，还发现男女之间的差异更大一些。 研究目的是比较摄入两种口服脂肪负荷（由长链甘油三酯（LCT）或中链甘油三酯（MCT）组成）期间的BHB曲线。LCT由具有12至20个碳原子的脂肪酸组成; MCT含有长度为8至12个碳原子的脂肪酸。如下所述，MCT的链长和其他性质的差异将使MCT比LCT更容易发生β-氧化。净效应应该是摄入MCT期间BHB的血浆水平高于LCT。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this pilot study is to determine whether Medium Chain Triglycerides (MCT) are better than Long Chain Triglycerides (LCT) in measuring fatty acid oxidation. Fatty acids are a source of energy and generally these fats are converted to acetyl coenzyme A in the mitochondria in the process of beta-oxidation. This assessment was done by other investigators using a fat tolerance test carried out over a 10 to 12 hour interval. Levels of beta-hydroxybutyrate (BHB) were measured and the area under the curve was calculated. The method was useful in detecting significant differences of about 15 to 20% between healthy subjects and those with familial combined hyperlipidemia. Also, somewhat greater differences were found between women and men. The study objective is to compare BHB curves during intake of two oral fat loads that consist of either long-chain triglycerides (LCT) or medium-chain triglycerides (MCT). LCT consists of fatty acids that have 12 to 20 carbon atoms; MCT contain fatty acids that are 8 to 12 carbon atoms in length. The difference in chain length and other properties of MCT, as detailed below, will allow MCT to undergo beta-oxidation to a greater extent than LCT. The net effect should be that plasma levels of BHB will be higher during the ingestion of MCT than LCT.