EXPERIMENTAL HUMAN INFECTION WITH HAEMOPHILUS DUCREYI IN PERSONS WHO ARE HIV

艾滋病毒感染者杜克雷嗜血杆菌的人类感染实验

• 批准号: 7379081
• 负责人: Stanley M. Spinola
• 金额: $ 0.29万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7379081
• 关键词: EXPERIMENTAL; HUMAN; INFECTION; HAEMOPHILUS; DUCREYI

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Haemophilus ducreyi is a bacterium that causes chancroid, a genital ulcer disease (GUD) that is common in many developing countries. Chancroid is a public health problem because persons with chancroid acquire the human immunodeficiency virus (HIV) at a rate that is higher than persons who do not have chancroid. Persons who are co-infected with HIV and chancroid have detectable HIV DNA or recoverable HIV in their genital ulcers and may transmit HIV more readily to their partners than persons infected with HIV alone. However, little is known about how the bacterial infection affects HIV replication in co-infected patients. The purpose of this protocol is to evaluate the safety of infecting selected, clinically stable, HIV seropositive individuals with H. ducreyi and to examine how H. ducreyi interacts with HIV. Our hypotheses are that local infection with H. ducreyi to the pustular stage of disease will be safe, and will not affect viral replication or CD4 counts in the systemic compartment, but will recruit latently infected memory CD4 cells and macrophages into the lesions, activate them, and cause them to produce virus locally. These studies will help us understand the biological mechanisms that allow co-infected persons to be more efficient transmitters of HIV than persons infected with HIV alone, establish which latently infected cells produce HIV upon immune stimulation, and provide a compelling scientific rationale to focus public health efforts on chancroid eradication in HIV endemic areas.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。杜氏嗜血杆菌是一种引起下巴的细菌，下巴是一种生殖器溃疡疾病，在许多发展中国家很常见。下巴是一个公共卫生问题，因为患有下巴的人感染人类免疫缺陷病毒(艾滋病毒)的比率高于没有下巴的人。与单独感染艾滋病毒的人相比，同时感染艾滋病毒和下巴的人在其生殖器溃疡中具有可检测到的艾滋病毒DNA或可恢复的艾滋病毒，并可能更容易将艾滋病毒传播给他们的伴侣。然而，关于细菌感染如何影响混合感染患者的艾滋病毒复制，人们知之甚少。该方案的目的是评估感染选定的临床稳定的HIV血清阳性个体的安全性，并检查H.ducreyi与HIV的相互作用。我们的假设是，局部感染H.ducreyi到疾病的脓疱期将是安全的，不会影响病毒复制或全身隔室中的CD4计数，但会将潜伏感染的记忆CD4细胞和巨噬细胞招募到病变中，激活它们，并使它们在局部产生病毒。这些研究将帮助我们理解允许混合感染者比单独感染艾滋病毒的人更有效地传播艾滋病毒的生物机制，确定哪些潜伏感染细胞在免疫刺激下产生艾滋病毒，并提供令人信服的科学理由，将公共卫生努力集中在根除艾滋病毒流行区的下巴上。