EXERCISE TESTING AND TRAINING FOR HAART TOXICITY

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。接受高效抗逆转录病毒疗法(HAART)治疗艾滋病毒感染的患者会受到药物的副作用，其中一些副作用可能是由于线粒体毒性。在这项研究中，我们试图确定运动试验是否可以用来识别和量化氧化代谢指标中反映的线粒体毒性。在前两个方案中，HIV感染患者将被分成三组：血乳酸正常的HAART组，血乳酸轻度升高的HAART组，以及从不接受HAART的HAART组。测定呼吸气体交换的运动试验和测定乳酸和丙酮酸浓度的血样。将测量身体成分，以确定人群的特征，并为瘦体重的锻炼措施编制指数。我们期望通过使用运动试验来确定一种简便的方法来监测患者的HAART相关线粒体毒性。在第二个方案中，接受HAART的患者还将接受运动测试和身体成分测量，并进行经皮肌肉活检。然后，他们将被随机分为运动训练组和不训练组，为期10周，之后将重复所有措施。由此，我们将从整体水平和肌肉细胞水平确定运动训练对HAART设置的氧化功能的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Patients on highly active antiretroviral therapy (HAART) for HIV infection are subject to side effects of drugs some of which may be due to mitochondrial toxicity. In this study we seek to identify whether exercise testing can be used to identify and quantify mitochondrial toxicity as reflected in measures of oxidative metabolism. In the first two protocols, HIV infected patients will be recruited in three groups: on HAART with normal blood lactate, on HAART with a mildly elevated blood lactate, and never on HAART. Exercise testing with measurement of respiratory gas exchange and blood sampling for determination of lactate and pyruvate concentrations. Body composition will be measured both to characterize the population and for indexing of exercise measures to lean mass. We expect to identify a facile approach to monitoring patients for HAART related mitochondrial toxicity by the use of exercise testing. In the second protocol patients on HAART will also undergo exercise testing and body composition measure, and will also have percutaneous mascle biopsy. They will then be randomized to exercise training or no training for 10 weeks, following which all the measures will be repeated. From this we will define the effects of exercise training on oxidative function at the level of both whole body level and muscle cell in the setting of HAART.