FAMILY STUDY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。这项研究是在华盛顿大都市地区的一组非裔美国人中调查高血压的遗传流行病学。具体目标是：i）招募并检查随机获得的350个AA家庭的基于人群的队列，每个家庭至少5人; ii）收集广泛的人口统计学数据（例如：预期随访、收入教育、吸烟、饮酒和家庭规模）和所有参与者的家族病史，iii）进行广泛的表型表征，包括：a）人体测量和相关变量：身高、体重、腰围和臀围、身体组成变量，包括脂肪量（FM）、去脂肪量（FFM），和体脂百分比（PBF）B）BP和相关的生理中间体，包括肾素-血管紧张素醛固酮系统（RAAS）的组分：血管紧张素原（AGT）、血管紧张素转换酶（ACE）和醛固酮。测量其他BP相关的中间表型，包括内皮素1（ET 1）和C-反应蛋白（CRP），c）糖尿病相关变量：所有参与者中的葡萄糖、C-肽和胰岛素。iv）评估BP、HTN与RAAS（ACE、AGT和血管紧张素II受体1型-AT 2 R1）的基因和其他选定的条件基因（包括上皮钠通道β亚基（ENaC）、内皮型一氧化氮合酶（eNOS 1、CRP、ET 1和β 2-肾上腺素能受体（ADRB 2））之间的关联和联系。 符合年龄要求的先证者（即，30-60岁）和家庭结构标准是从随机选择的，主要是来自DC大都市区的非洲裔美国人社区中确定的，之后先证者和同意的家庭成员被安排在GCRC的诊所预约。要求参加者在诊所预约的前一天晚上禁食至少8小时。在GCRC，获得书面知情同意书，然后获得人口统计学数据和病史。人体测量、血压和身体成分测量是使用标准化方法进行的。从每个参与者获得空腹血液样品，其包括两个IOml EDTA管用于血浆和血沉棕黄层（DNA的来源），一个15 ml红盖管用于血清，以及一个5 ml灰盖管用于空腹葡萄糖测量。还从所有参与者获得现场尿液。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The study is examining the genetic epidemiology of hypertension in a cohort of African Americans in the Washington, DC metropolitian area. The specific goals are to i) Enroll and examine a randomly acertained population-based cohort of 350 AA families with a minimum of 5 persons per family ii) Collect extensive demographic data (eg. adddress for anticipated follow-up, income education, smoking, drinking and household size) and family medical history on all participants , iii) Perform extensive phenotypic characterization, including: a) anthropometric and related variables: height, weight, waist and hip circumference, body composition variables, including fat mass (FM), fat free mass (FFM), and percent body fat (PBF) using Bioelectric Impedance Analyzer (BIA) in all participants b) BP and related physiologic intermediates including components of the renin-angiotensin aldosterone system (RAAS): angiotensinogen (AGT), angiotensin converting enzyme (ACE) and aldosterone. Measure other BP related intermediate pheotypes including endothelin 1 (ET1) and C-reactive protein(CRP), c) diabetes related variables: glucose, c-peptide and insulin in all participants. iv) Evaluate the association and linkage between BP, HTN and genes of the RAAS (ACE, AGT and angiotensin II receptor type 1- AT2R1) and other selected condidate genes including epithelial sodium channel beta subunit (ENaC), endothelial nitic oxide synthases (eNOSs\, CRP, ET1, and beta2-adrenergic receptor (ADRB2). Probands satisfying the age requirement (i.e., 30-60 years) and family structure criteria are identified from randomly selected, predominantly African American communities from the DC metropolitan area, after which the proband and consenting family members are scheduled for a clinic appointment in the GCRC. Participants are requested to fast for a minimum of eight hours on the night preceding the clinic appointment. At the GCRC, written informed consent is obtained following which demographic data and a medical history are obtained. Anthropometric, blood pressure and body composition measurements are done using standardized methods. Fasting blood samples are obtained from each participant comprising two 10ml EDTA tubes for plasma and buffy coat (source of DNA), one 15ml red top for serum and a 5 ml gray top for fasting glucose measurement. Spot urine are also obtained from all participants.