MUSCLE BIOPSY FRAIL VS NON-FRAIL

肌肉活检虚弱与非虚弱

• 批准号: 7377338
• 负责人: ANNE M KENNY
• 金额: $ 0.26万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7377338
• 关键词: MUSCLE; BIOPSY; FRAIL; VS; NON

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Although frailty has been difficult to define, Fried and her colleagues have established criteria for frailty based on physical ad psychological characteristics. These characteristics include unintentional weight loss (10 or more pounds per year), self-reported exhaustion, weakness as measured by grip strength, slow walking speed, and low physical activity (Fried et al., 2001). With a framework to examine frailty, we propose to explore more fully the pathophysiology of sarcopenia in frail and non-frail older individuals. In Frontera et al. 2000 and Balagopal et al. 1997, the authors hypothesized that sarcopenia largely results from the decreased ability for the replacement of dysfunctional contractile proteins within the myofilament lattice. Exploiting the signal in Second Harmonic Imagine Microscopy (SHIM), which is derived from and sensitive to the local density and alignment of contractile proteins within muscle sarcomeres, this new mode of non-linear laser-scanning microscopy will allow quantitative analysis of both the histology and molecular structure of completely native, intact muscle tissue. Thus far, few studies to date have provided extensive and quantitative ultrastructural examination of muscle from very old individuals (Frontera et al. 2000). Fiatarone Singh and colleagues, reported data outlining aspects of muscle damage in frail elders using electron microscopy, which has limitations in comparison to SHIM.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。虽然虚弱很难定义，但弗里德和她的同事们已经根据身体和心理特征建立了虚弱的标准。这些特征包括无意的体重减轻（每年10磅或更多磅）、自我报告的疲惫、通过握力测量的虚弱、缓慢的步行速度和低体力活动（Fried等人，2001年）。通过一个框架来检查虚弱，我们建议更充分地探索虚弱和非虚弱老年人的肌肉减少症的病理生理学。在Frontera et al. 2000和Balagopal et al. 1997中，作者假设肌肉减少症主要是由于肌丝晶格内功能障碍性收缩蛋白的替代能力降低所致。利用二次谐波成像显微镜（SHIM）中的信号，该信号来源于肌肉肌节内收缩蛋白的局部密度和排列，这种新的非线性激光扫描显微镜模式将允许对完全天然的完整肌肉组织的组织学和分子结构进行定量分析。到目前为止，很少有研究提供了广泛的和定量的超微结构检查肌肉从非常老的人（Frontera等，2000年）。Fiatarone Singh及其同事报告了使用电子显微镜概述虚弱老年人肌肉损伤方面的数据，与SHIM相比，电子显微镜具有局限性。