EFFECT OF INSULIN ON PROTEIN SYNTHESIS IN PUBERTAL CHILDREN

胰岛素对青春期儿童蛋白质合成的影响

• 批准号: 7375323
• 负责人: T HASTINGS WILSON
• 金额: $ 0.04万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7375323
• 关键词: EFFECT; INSULIN; PROTEIN; SYNTHESIS; PUBERTAL

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is to determine the importance of insulin in maintaining protein synthesis in children with Type 1 Diabetes. It is the hypothesis of the study that the requirement of insulin for protein metabolism is different from the insulin requirement for carbohydrate metabolism and that maintaining children in glycemic control may not provide sufficient insulin for anabolic responses of protein metabolism associated with growth. We will address this hypothesis by examining the anabolic effect of insulin in pubertal children with type 1 diabetes. Whole-body protein metabolism will be determined in the post absorptive state without insulin and then with the provision of insulin, amino acids, and glucose. The acute effect of insulin on whole-body protein metabolism will be assessed from the kinetics of L-[1-13C] leucine. By determining the protein balance in the presence of insulin at levels comparable to those achieved during normal feeding, we will be able to ascertain whether the children are sufficient protein balance to maintain growth.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。本研究的目的是确定胰岛素在维持1型糖尿病儿童蛋白质合成中的重要性。本研究的假设是，蛋白质代谢对胰岛素的需求不同于碳水化合物代谢对胰岛素的需求，维持儿童血糖控制可能无法为与生长相关的蛋白质代谢的合成代谢反应提供足够的胰岛素。我们将通过检查胰岛素在青春期1型糖尿病儿童中的合成代谢作用来解决这一假设。全身蛋白质代谢将在没有胰岛素的吸收后状态下测定，然后在提供胰岛素、氨基酸和葡萄糖时测定。胰岛素对全身蛋白质代谢的急性影响将从L-[1-13C]亮氨酸的动力学来评估。通过测定在胰岛素存在下的蛋白质平衡水平与正常喂养时达到的水平相当，我们将能够确定儿童是否有足够的蛋白质平衡来维持生长。