A characterisation of last order interneurons of the rodent spinal cord with specific focus on their roles in the control of locomotor activity

啮齿动物脊髓末阶中间神经元的表征，特别关注它们在控制运动活动中的作用

• 批准号: BB/E019803/1
• 负责人: Gareth Miles
• 金额: $ 47.33万
• 依托单位: University of St Andrews
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE019803%2F1
• 关键词: characterisation; last; order; interneurons; rodent

It has been known since the early 1900's that networks of neurons within the mammalian spinal cord control locomotion. However, our knowledge of these neuronal networks is very limited. In order to design treatments for paralysing injuries or diseases of the spinal cord, we need to gain a better understanding of the networks of spinal neurons that control movement. This initially requires the identification of neurons which make up these networks. Following their identification we need to study the individual properties of these neurons and how it is that they connect to each other to produce the activity which controls movement. The proposed study aims to provide some of this critical information. Molecular techniques will be used to identify specific populations of neurons in the spinal cord. Using these techniques we will be able to concentrate on 'last order interneurons' which communicate directly with the neurons that send the final message to muscles to contract (motoneurons). Following their identification, last order interneurons will be studied during locomotor activity in isolated preparations of the spinal cord obtained from mice. This will enable us to investigate the roles that specific groups of last order interneurons play in the production of locomotor activity. As outlined above this information is critical toward our understanding of the neural control of movement and ultimately towards the design of treatment strategies for injury or disease which affects the spinal cord.

早在20世纪初，S就知道哺乳动物脊髓内的神经元网络控制着运动。然而，我们对这些神经元网络的了解非常有限。为了设计脊髓瘫痪损伤或疾病的治疗方法，我们需要更好地了解控制运动的脊髓神经元网络。这首先需要识别组成这些网络的神经元。在确认它们之后，我们需要研究这些神经元的个体属性，以及它们是如何相互连接以产生控制运动的活动的。拟议的研究旨在提供其中一些关键信息。分子技术将被用来识别脊髓中特定的神经元群体。使用这些技术，我们将能够专注于“最后一级中间神经元”，它们直接与将最终信息发送给肌肉收缩的神经元(运动神经元)进行交流。在鉴定后，将在小鼠脊髓的分离标本中研究最后一级中间神经元在运动活动中的作用。这将使我们能够调查特定的末级中间神经元组在产生运动活动中所扮演的角色。如上所述，这些信息对于我们理解运动的神经控制，最终对于设计影响脊髓的损伤或疾病的治疗策略是至关重要的。

项目成果

A cluster of cholinergic premotor interneurons modulates mouse locomotor activity.

• DOI: 10.1016/j.neuron.2009.10.017
• 发表时间: 2009-12-10
• 期刊: NEURON
• 影响因子: 16.2
• 作者: Zagoraiou, Laskaro;Akay, Turgay;Martin, James F.;Brownstone, Robert M.;Jessell, Thomas M.;Miles, Gareth B.
• 通讯作者: Miles, Gareth B.

Glial-derived adenosine modulates spinal motor networks in mice.

胶质细胞来源的腺苷调节小鼠的脊髓运动网络。

• DOI: 10.1152/jn.00513.2011
• 发表时间: 2012
• 期刊: Journal of neurophysiology
• 影响因子: 2.5
• 作者: Witts EC