PREVALENCE OF GSTM1, GSTT1, AND GSTP1B AMONG HISPANICS IN PUERTO RICO

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。肺癌仍然是美国和中华人民共和国的一个主要公共卫生问题。肺癌的危险因素是吸烟、环境因素和遗传易感性。在遗传易感性方面，虽然80%至90%的肺癌患者是吸烟者，但只有10%的吸烟者会患上肺癌。对香烟烟雾中存在的致癌物的敏感性差异通常被认为是由个体遗传背景的差异造成的。谷胱甘肽S-转移酶（GST）是哺乳动物中用于不同异生物质解毒的诱导酶家族。一些研究表明，缺乏GSTM 1，GSTT 1和GSTP 1b与肺癌的患病率和进展有关。这些研究大多集中在白人和非裔美国人人群中，关于西班牙裔人群的信息有限。因此，作为填补西班牙裔人群中这一空白的第一步，我们建议获得这些基因在普通人群中的患病率。这些信息将使我们能够更好地设计未来对肺癌干预和基因-环境相互作用的分析研究。 为了实现本申请的目的，我们将追求以下具体目标：1.使用唾液样本筛选来自波多黎各的250名西班牙裔受试者，并确定GSTM 1（null）、GSTT 1（null）和GSTP 1b（ile/瓦尔）的患病率。2.评估已知危险因素（吸烟、职业危害等）的分布情况。肺癌的治疗方法3.通过人口统计学特征（即性别、年龄等）描述样本人群的特征。以及生活方式和健康相关变量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Lung cancer stills a major public health problem in the U.S. and P.R. Risk factors for lung cancer are cigarette smoking, environmental factors, and genetic predisposition. In relation to the genetic predisposition, although 80 to 90% of patients with lung cancer are smokers only 10% of smokers will develop lung cancer. Susceptibility differences to carcinogens present in cigarette smoke are generally thought to result from differences in the genetic background of individuals. Glutathione S-transferases (GSTs) are a family of inducible enzymes used in the detoxification of different xenobiotics in mammals. Several studies have shown an association for the lack of GSTM1, GSTT1, and the presence of GSTP1b with prevalence and progression of lung cancer. Most of these studies have focused in the Caucasian and African American populations, and there is limited information regarding the Hispanic population. Thus, as a first step to fill this void in the Hispanic population we proposed to obtain the prevalence of these genes in the general population. This information will allow us to better design future analytical studies on lung cancer interventions and gene-environment interactions. To accomplish the objective of this application, we will pursue the following specific aims: 1. To screen 250 Hispanic subjects from Puerto Rico using saliva samples and determine the prevalence of GSTM1 (null), GSTT1 (null), and GSTP1b (ile/val). 2. To evaluate the distribution of known risk factors (smoking, occupational hazard, etc.) for lung cancer by GST categories. 3. To characterize the sample population by demographic characteristics (i.e. gender, age, etc.) as well as by lifestyle and health related variables.