NE COBRE: MICROSCOPY CORE RESEARCH FACILITY

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。生物技术中心(UNL)的显微镜核心研究机构积极参与了许多利用显微镜进行的研究，包括由NCV成员和同事进行的研究。在2005-2006财年(从2005年7月1日至2006年1月31日)，来自NCV成员和助理(本财年迄今有19名教员)实验室的博士后研究人员、工作人员以及研究生/本科生大量使用了显微镜核心。NCV成员对这一核心的主要用途与免疫荧光共聚焦显微镜有关，用于鉴定、定位和表征病毒抗原，一些用途涉及通过扫描或透射电子显微镜进行超微结构和形貌研究，以可视化、鉴定、定位和表征病毒颗粒/组分。具有5个通道和6条激光线路、可控制温度和二氧化碳水平的倒置共焦，为NCV成员和伙伴提供了一种有用的活细胞成像工具，并在本财年得到了广泛应用。此外，该核心还在倒置荧光显微镜上安装了高灵敏度的CCD摄像头，用户无需使用共焦系统即可采集荧光或传输图像。该中心还帮助或参与了NCV成员在今年提交的几项新的赠款提案。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Microscopy Core Research Facility of the Center for Biotechnology-UNL, has been actively involved in many studied utilizing microscopy, including those conducted by NCV members and associates. During FY2005-2006 (from July 1, 2005 to January 31, 2006), the Microscopy Core has been heavily utilized by postdoctoral researchers, staff, and graduate/undergraduate students from laboratories of the NCV members and associates (19 faculty members to date in this FY). The main usages of this core by NCV members are related to immunofluorescence confocal microscopy for identification, localization and characterization of viral antigens, and some uses are related to ultrastructural and topographic studies by scanning or transmission electron microscopy, for visualization, identification, localization and characterization of viral particles/component. A inverted confocal with 5 channels and 6 laser lines and controlled temperature and CO2 level, has provided NCV members and associates with a useful tool for live cell imaging and has been heavily utilized in this FY. In addition, the core has installed a high sensitivity CCD camera on an inverted fluorescence microscope for users to collect florescence or transmit images without using confocal system. The Core has also helped or participated in several new grant proposals submitted by NCV members in this year.