RELEASE TIME TO STUDY THE PATHWAY OF 5'-NUCLEOTIDASE IN DICTYOSTELIUM

释放时间来研究盘基菌中 5-核苷酸酶的通路

• 批准号: 7381680
• 负责人: MUATASEM UBEIDAT
• 金额: $ 0.79万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381680
• 关键词: RELEASE; TIME; STUDY; PATHWAY; NUCLEOTIDASE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Release time is given for the study of "Tagging and Characterization of Genes in the Signal Transduction Pathway of 5'-Nucloetidase in Dictyostelium." This will allow research development which will coincide with the mini-grant project currently being funded by INBRE. Dr. Ubeidat will design several experiments and approaches to tag genes that are part of the signal transduction pathway of 5nt. Understanding the function of 5'-nucleotidase gene and its protein product require both in vivo and in vitro analysis of various mutants in the signal transduction pathway of the gene. His goal is to identify such mutants that could be used to identify functional genes that are required for proper operation of the gene and necessary for the proper differentiation of the cells. Using REMI and other methods like microarray and RNAi to analyze gene expression will give more information about the specific function and role in Dictyostelium and probably more information to help understand the functions in other organisms like humans.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。为“盘基钢菌5′-核苷酶信号转导通路基因的标记与表征”的研究提供了释放时间。这将使研究发展与目前由INBRE资助的小型赠款项目相吻合。Ubeidat博士将设计几个实验和方法来标记基因，这些基因是5nt信号转导途径的一部分。了解5′-核苷酸酶基因及其蛋白产物的功能，需要对该基因信号转导途径中的各种突变体进行体内和体外分析。他的目标是识别这样的突变体，这些突变体可以用来识别基因正常运作所需的功能基因，以及细胞正常分化所必需的功能基因。使用REMI和其他方法如微阵列和RNAi来分析基因表达将提供更多关于盘基骨柱的特定功能和作用的信息，并可能有助于了解其他生物如人类的功能。