RELEASE TIME TO STUDY THE PATHWAY OF 5'-NUCLEOTIDASE IN DICTYOSTELIUM

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。发布时间是为研究“标记和表征的基因在信号转导途径的5 '-核磷脂酶在网柄藻。“这将使研究开发与INBRE目前资助的小额赠款项目相吻合。Ubeidat博士将设计几个实验和方法来标记基因，这些基因是5 nt信号转导途径的一部分。了解5 '-核苷酸酶基因及其蛋白产物的功能需要对该基因信号转导途径中的各种突变体进行体内和体外分析。他的目标是鉴定这样的突变体，这些突变体可用于鉴定基因正常运作所需的功能基因，以及细胞正常分化所必需的功能基因。使用REMI和其他方法如微阵列和RNAi来分析基因表达将提供更多关于网骨藻的特定功能和作用的信息，并可能提供更多信息来帮助理解其他生物如人类的功能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Release time is given for the study of "Tagging and Characterization of Genes in the Signal Transduction Pathway of 5'-Nucloetidase in Dictyostelium." This will allow research development which will coincide with the mini-grant project currently being funded by INBRE. Dr. Ubeidat will design several experiments and approaches to tag genes that are part of the signal transduction pathway of 5nt. Understanding the function of 5'-nucleotidase gene and its protein product require both in vivo and in vitro analysis of various mutants in the signal transduction pathway of the gene. His goal is to identify such mutants that could be used to identify functional genes that are required for proper operation of the gene and necessary for the proper differentiation of the cells. Using REMI and other methods like microarray and RNAi to analyze gene expression will give more information about the specific function and role in Dictyostelium and probably more information to help understand the functions in other organisms like humans.