ROLE OF COHESION IN APOPTOTIC CELL DEATH

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。细胞凋亡是细胞死亡的一种活跃形式，在所有后生动物的发育过程中起着至关重要的作用。最近的研究表明，粘附素是一种调节姐妹染色单体凝聚力的蛋白质复合体，在细胞凋亡中发挥着重要作用。粘附素RAD21的抑制降低了细胞的增殖能力，增加了细胞的凋亡水平。RAD21还被发现在前列腺癌和乳腺癌细胞中过表达。了解粘附素在细胞凋亡中的作用将提高我们对癌症生物学的认识。我们最近的研究表明，酵母中粘附素的突变会导致细胞凋亡。外界刺激，如过氧化氢，会导致粘附素从细胞核转移到线粒体，这在细胞凋亡中起着重要作用。我们建议以芽殖酵母为模型系统，研究粘附素在细胞凋亡中的作用。我们的目标是了解粘附素的功能，因为它与调控复杂的细胞凋亡过程的分子机制有关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Apoptosis is an active form of cell death that plays a crucial role in the development of all metazoans. Recent studies show that cohesin, a protein complex that regulates sister chromatid cohesion, plays an important role in apoptosis. Suppression of cohesin RAD21 reduces the ability for cell proliferation and increases the level of apoptosis. RAD21 is also found to be overexpressed in prostate and breast cancer cells. Understanding of role of cohesin in apoptosis will enhance our knowledge of cancer biology. Our recent studies showed that mutations of cohesin in yeast cause apoptotic cell death. External stimuli, such as hydrogen peroxide, causes cohesin translocated from nucleus to mitochondria, which plays an important role in apoptosis. We propose here to study the function of cohesin in apoptosis using budding yeast as model system. Our goal is to understand the function of cohesin as it relates to the molecular mechanisms that regulate the complex process of apoptosis.