PUI RESEARCH-TOUGALOO-HENNINGTON

PUI 研究-图加卢-亨宁顿

• 批准号: 7381620
• 负责人: BETTYE S HENNINGTON
• 金额: $ 17.77万
• 依托单位: UNIVERSITY OF SOUTHERN MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381620
• 关键词: PUI; RESEARCH; TOUGALOO; HENNINGTON

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the model eukaryote Saccharomyces cerevisiae, cell cycle events are coordinated with constitutive functions like energy generation and duplication of protein mass. The latter processes are stimulated by the Rap1p/Gcr1p/Gcr2p complex, which activates transcription of the genes required for rapid growth; this includes glycolytic and ribosomal protein genes, which are among the most heavily transcribed in the cell. In vivo 32P labeling has shown that Rap1p, Gcr1p, and Gcr2p are all phosphoproteins. Mapping the exact sites of phosphorylation in these proteins would represent an important advance in our understanding of the signaling cascade(s) that regulates the Rap1 activation complex. Mass spectrometry (LC-MS/MS) will be used to analyze phosphorylation sites in these factors.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。在真核生物酿酒酵母模型中，细胞周期事件与能量产生和蛋白质质量复制等基本功能相协调。后一个过程受到 Rap1p/Gcr1p/Gcr2p 复合物的刺激，该复合物激活快速生长所需基因的转录；这包括糖酵解和核糖体蛋白基因，它们是细胞中转录最频繁的基因。体内32P标记显示Rap1p、Gcr1p和Gcr2p都是磷蛋白。绘制这些蛋白质中磷酸化的确切位点将代表我们对调节 Rap1 激活复合物的信号级联的理解取得了重要进展。质谱 (LC-MS/MS) 将用于分析这些因子中的磷酸化位点。