PUI RESEARCH-TOUGALOO-HENNINGTON

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。在真核生物酿酒酵母模型中，细胞周期事件与能量产生和蛋白质质量复制等结构性功能相协调。后一种过程由Rap1p/Gcr1p/Gcr2p复合体刺激，它激活快速生长所需基因的转录；这包括糖酵解和核糖体蛋白基因，它们是细胞中转录最多的基因之一。体内~(32)P标记表明，Rap1p、Gcr1p和Gcr2p都是磷蛋白。绘制这些蛋白质中磷酸化的确切位置将代表着我们对调节Rap1激活复合体的信号级联(S)的理解取得了重要进展。将使用LC-MS/MS来分析这些因子中的磷酸化位点。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the model eukaryote Saccharomyces cerevisiae, cell cycle events are coordinated with constitutive functions like energy generation and duplication of protein mass. The latter processes are stimulated by the Rap1p/Gcr1p/Gcr2p complex, which activates transcription of the genes required for rapid growth; this includes glycolytic and ribosomal protein genes, which are among the most heavily transcribed in the cell. In vivo 32P labeling has shown that Rap1p, Gcr1p, and Gcr2p are all phosphoproteins. Mapping the exact sites of phosphorylation in these proteins would represent an important advance in our understanding of the signaling cascade(s) that regulates the Rap1 activation complex. Mass spectrometry (LC-MS/MS) will be used to analyze phosphorylation sites in these factors.