PUI RESEARCH-TOUGALOO-HENNINGTON

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。在模型真核生物酿酒酵母中，细胞周期事件与组成性功能如能量产生和蛋白质质量复制相协调。后一个过程由Rap 1 p/Gcr 1 p/Gcr 2 p复合物刺激，该复合物激活快速生长所需的基因的转录;这包括糖酵解和核糖体蛋白基因，它们是细胞中转录最多的基因之一。体内~（32）P标记表明，Rap 1 p、Gcr 1 p和Gcr 2 p都是磷蛋白。绘制这些蛋白质中磷酸化的确切位点将代表我们对调节Rap 1激活复合物的信号级联的理解的重要进展。将使用质谱法（LC-MS/MS）分析这些因子中的磷酸化位点。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the model eukaryote Saccharomyces cerevisiae, cell cycle events are coordinated with constitutive functions like energy generation and duplication of protein mass. The latter processes are stimulated by the Rap1p/Gcr1p/Gcr2p complex, which activates transcription of the genes required for rapid growth; this includes glycolytic and ribosomal protein genes, which are among the most heavily transcribed in the cell. In vivo 32P labeling has shown that Rap1p, Gcr1p, and Gcr2p are all phosphoproteins. Mapping the exact sites of phosphorylation in these proteins would represent an important advance in our understanding of the signaling cascade(s) that regulates the Rap1 activation complex. Mass spectrometry (LC-MS/MS) will be used to analyze phosphorylation sites in these factors.