VERMONT IMMUNOBIOL/INFECTIOUS DIS CTR: MIICROARRAY & BIOINFORMATIC ANALYSIS CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。微阵列核心设施采用Affymetrix基因芯片2500系统，包括杂交站640，流体站400和扫描仪2500。Agilant生物分析仪2100也可用于RNA分析。基因芯片表达分析探针阵列从Affymetrix购买。微阵列核心还配备了各种数据分析，网络和数据库管理工具。数据分析使用Microarray Suite， DMT, Micro DB， genesspring和Gene Sifter软件包。Microarray Core提供RNA表达分析服务，包括RNA的质量测试、与GeneChip探针阵列的杂交、图像处理、原始数据分析、数据传递和访问生物信息学工具，这些都由生物信息学Core管理。用户支持可用于使用各种软件包进行目标准备和数据分析。生物信息学核心设施提供一名Unix系统管理员和两名程序员的支持。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Microarray core facility utilizes the Affymetrix GeneChip 2500 system that includes Hybridization Station 640, Fluidics Station 400 and Scanner 2500. An Agilant bioanalyzer 2100 is also available for RNA analysis. GeneChip expression analysis probe arrays are purchased from Affymetrix. The microarray core is also equipped with a variety of tools for data analysis, networking and database management. Data are analyzed using the Microarray Suite, DMT, Micro DB, GeneSpring and Gene Sifter software packages. The Microarray Core provides RNA expression-profiling services including quality testing of RNA, hybridization to GeneChip probe arrays, image processing, primary data analysis, data delivery, and access to Biomformatics tools, which are managed by the Bioinformatics Core. User support is available for target preparation and data analysis using various software packages. The Bioinformatics core facility provides support with a Unix system administrator and two programmers.