VERMONT IMMUNOBIOL/INFECTIOUS DIS CTR: MICROARRAY & BIOINFORMATIC ANALYSIS CORE

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 微阵列核心设施利用Affyscore GeneChip 2500系统，该系统包括杂交站640、射流站400和扫描仪2500。也可使用生物分析仪2100进行RNA分析。GeneChip表达分析探针阵列购自Affytechnology。微阵列核心还配备了用于数据分析、网络和数据库管理的各种工具。使用Microarray Suite、DMT、Micro DB、GeneSpring和Gene Sifter软件包分析数据。微阵列核心提供RNA表达谱分析服务，包括RNA质量检测、与基因芯片探针阵列杂交、图像处理、原始数据分析、数据交付和访问生物信息学工具，这些服务由生物信息学核心管理。使用各种软件包，为目标准备和数据分析提供用户支持。生物信息学核心设施提供Unix系统管理员的支持 两个程序员。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Microarray core facility utilizes the Affymetrix GeneChip 2500 system that includes Hybridization Station 640, Fluidics Station 400 and Scanner 2500. An Agilant bioanalyzer 2100 is also available for RNA analysis. GeneChip expression analysis probe arrays are purchased from Affymetrix. The microarray core is also equipped with a variety of tools for data analysis, networking and database management. Data are analyzed using the Microarray Suite, DMT, Micro DB, GeneSpring and Gene Sifter software packages. The Microarray Core provides RNA expression-profiling services including quality testing of RNA, hybridization to GeneChip probe arrays, image processing, primary data analysis, data delivery, and access to Biomformatics tools, which are managed by the Bioinformatics Core. User support is available for target preparation and data analysis using various software packages. The Bioinformatics core facility provides support with a Unix system administrator and two programmers.