COBRE: LSU HSC: CELL & MOLECULAR CORE

COBRE：路易斯安那州立大学 HSC：细胞

• 批准号: 7382068
• 负责人: Emel Songu-Mize
• 金额: $ 11.95万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7382068
• 关键词: COBRE; LSU; HSC; CELL; MOLECULAR

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cell and Molecular Core Directors: Emel Songu Mize, Ph.D. and Stephen M. Lanier, Ph.D. This core has three major support missions: Cell Culture; Proteomics; and Imaging. The facility is staffed by Marella Kuriakose, M.S., Jesse Guidry, B.S. (Instructor)and Olha Nichols, M.S. CELL CULTURE The core supplies primary cultures for COBRE investigators. This includes rat aortic smooth muscle cells (users: Drs. Catling, Park, Wu, Songu Mize, Park), mouse macrophage cultures (user: Dr. Boulares), rat neonatal cardiac cell cultures (user: Drs. Park and Wu), HUVEC cultures (users: Drs. Park and Boulares). In addition to the above, the Cell Culture core facility developed additional cell lines of mouse mesenteric smooth muscle cells (Drs. Lucchesi, Catling, Songu-Mize), embryonic fibroblasts (Dr. Boulares) and is in the process of establishing adult rat cardiac myocytes in culture (Drs. Lucchesi, Wu, Songu-Mize). In order to respond to the expanding needs of the COBRE faculty Ms. Olga Nichols, who has expertise in confocal microscopy, histology and histopathology, came on board last year and is sharing the expanding responsibilities of maintaining and providing cultured cells to the investigators and overseeing the Imaging component of the Core. The COBRE investigators are provided a weekly report of available cell lines, passage numbers, both the frozen supplies and thriving cultures. They in turn make their request for the following week. Ms. Kuriakose delivers to each investigator what they need. PROTEOMICS AND IMAGING In July 2004, the proteomics core installed an Ettan Spot Handling Workstation. This instrument automates the processing of proteomic samples from 2D gels unto the ultimate processing via mass spectrometry. In April 2005, the molecular core also brought online an IcylerIQ real-time PCR machine and an Experion Electrophoresis station for the analysis of DNA, RNA and protein. Jesse Guidry continues to develop and foster proteomics projects through collaboration (LTQ analysis of phosphoproteome at Children's Hospital, SELDI analysis of human serum at University of Missouri, Kansas City). Work continues in the vascular smooth muscle characterization. In addition, the core is also aiding in the proteomic analysis of fusion protein enrichment, renal artery, brain proteomics, galectins, phosphoproteome, interactomes and others in conjunction with COBRE investigators. A list of abstracts and a manuscript that resulted from the use of Cell Culture Core cell lines is attached: Abstracts 1) Hamid Boulares, Karine Oumouna-Benachour, Manika Das, and Kirk Hutchinson: PARP-1 inhibition promotes factors of atherosclerotic plaque stability in a mouse model of atherosclerosis and prevents death of vascular cells in vitro. Vascular Biology and Medicine 2005: From Molecules to Man; Chicago June 16-19, 2005 2) Hoffmann SE, Zhang Z, Songu-Mize E: Effect of cyclic stretch on TRPC expression and calcium mobilization. Experimental Biology, April 2-6, 2005 San Diego. 3) Hoffmann SE, Kuriakose M, Songu-Mize E: Effect of cyclic stretch on TRPC4 protein and gene expression in vascular smooth muscle cells. Submitted to American Heart Association, High Blood Pressure Council, 2005 4) Kevin Lord, Ryan Reed, Alissa Hicks, Pamela Lucchesi and Kurt Varner. Role of Oxidative stress in methamphetamine-induced cardiac dysfunction. Keystone: Molecular Biology of Cardiac Diseases and Regeneration April 3-8th, 2005. 5) Violaine Simon, Jessie Guidry, Chau-Wen Chou and Stephen M Lanier. Identification of accessory proteins complexing with the third intracellular loop of M2 muscarinic receptors. Experimental Biology, April 2-6, 2005 San Diego. 6) RE Reed, HH Hughie LJ Dell?Italia and PA Lucchesi. Increased peroxynitrite production is a key mediator of LV extracellular matrix remodeling in response to acute volume overload. Keystone: Molecular Biology of Cardiac Diseases and Regeneration April 3-8th, 2005. Manuscripts: 4) Pullikuth, A. K., McKinnon, E., Schaeffer, H. J., and Catling, A. D. The MEK1 scaffolding protein MP1 regulates cell spreading by integrating PAK1 and Rho signals. Mol. Cell. Biol., in press.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。细胞和分子核心主管：Emel Songu Mize博士和Stephen M.Lanier博士。该核心有三个主要的支持任务：细胞培养、蛋白质组学和成像。该设施的工作人员为Marella Kuriakose，M.S.，Jesse Guidry，B.S.和Olha Nichols，M.S.。这包括大鼠主动脉平滑肌细胞(用户：Catling，Park，Wu，Songu Mize，Park)、小鼠巨噬细胞培养(用户：Boulares博士)、大鼠新生心肌细胞培养(用户：Park和Wu博士)、HUVEC培养(用户：Park和Boulares博士)。此外，细胞培养核心设施还开发了额外的小鼠肠系膜平滑肌细胞系(Lucchesi、Catling、Songu-Mize博士)、胚胎成纤维细胞(Boulares博士)，并正在建立成年大鼠心肌细胞的培养过程(Lucchesi、Wu、Songu-Mize博士)。为了应对Cobre教职员工日益增长的需求，在共焦显微镜、组织学和组织病理学方面拥有专业知识的Olga Nichols女士于去年加入公司，她将分担不断扩大的责任，负责维护和向研究人员提供培养细胞，并监督Core的成像部分。科布雷的调查人员每周都会收到一份关于可用细胞系、传代数量、冷冻供应和蓬勃发展的培养物的报告。他们依次提出了下周的请求。Kuriakose女士向每位调查员传达了他们所需要的信息。蛋白质组学和成像2004年7月，蛋白质组学核心安装了Ettan Spot处理工作站。该仪器自动处理蛋白质组样品，从2D凝胶到最终通过质谱学处理。2005年4月，分子核还推出了一台IcylerIQ实时PCR机和一台Experion电泳站，用于DNA、RNA和蛋白质的分析。Jesse Guidry继续通过合作开发和促进蛋白质组学项目(儿童医院的磷酸蛋白质组的LTQ分析，密苏里大学堪萨斯城大学的SELDI分析)。关于血管平滑肌特性的工作仍在继续。此外，该核心还与Cobre研究人员合作，帮助进行融合蛋白浓缩、肾动脉、脑蛋白质组学、Galectins、磷蛋白质组、相互作用和其他蛋白质组分析。摘要列表和使用细胞培养核心细胞系产生的手稿附：摘要1)Hamid Boulares、Karine Oumouna-Benachour、Manika Das和Kirk Hutchinson：PARP-1抑制促进动脉粥样硬化小鼠模型中动脉粥样硬化斑块稳定的因素，并防止体外血管细胞死亡。血管生物学与医学2005：从分子到人类；芝加哥，2005年6月16-19日2)Hoffmann SE，Zhang Z，Songu-Mize E：周期性拉伸对TRPC表达和钙动员的影响。实验生物学，2005年4月2日至6日，圣地亚哥。3)Hoffmann SE，Kuriakose M，Songu-Mize E：周期性拉伸对血管平滑肌细胞TRPC4蛋白和基因表达的影响。提交给美国心脏协会，高血压委员会，2005年4)凯文·洛德、瑞安·里德、艾丽莎·希克斯、帕梅拉·卢切西和库尔特·瓦尔纳。氧化应激在甲基苯丙胺所致心功能障碍中的作用。重点：心脏疾病的分子生物学与再生，2005年4月3日至8日。5)维奥莱恩·西蒙、杰西·古德里、周周文和斯蒂芬·M·拉尼尔。M2受体胞内第三环复合辅助蛋白的鉴定。实验生物学，2005年4月2日至6日，圣地亚哥。6)RE Reed、HH Hughie LJ Dell？Italia和PA Lucchesi。过氧亚硝酸盐的产生增加是急性容量超负荷后左室细胞外基质重塑的关键介质。重点：心脏疾病的分子生物学与再生，2005年4月3日至8日。手稿：4)Pullikuth，A.K.，McKinnon，E.，Schaeffer，H.J.和Catling，A.D.MEK1支架蛋白MP1通过整合PAK1和Rho信号来调节细胞扩散。摩尔。牢房。Biol.，正在印刷中。